Protein Blotting and Detection

Methods and Protocols

  • Biji T. Kurien
  • R. Hal Scofield

Part of the Methods in Molecular Biology book series (MIMB, volume 536)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Harry Towbin
    Pages 1-3
  3. Biji T. Kurien, R. Hal Scofield
    Pages 9-22
  4. Graeme Lowe
    Pages 67-73
  5. Rafael F. Pont-Lezica
    Pages 75-88
  6. S. Sumi, A. Mathai, V.V. Radhakrishnan
    Pages 89-93
  7. Dalit Talmi-Frank, Charles L. Jaffe, Gad Baneth
    Pages 103-113
  8. Engelbert Buxbaum
    Pages 115-128
  9. Ulrich Canzler, Holger Bartsch, Kai Großmann, Werner Lehmann, Karsten Conrad, Biji T. Kurien et al.
    Pages 129-137
  10. Joon-Yong Chung, Stephen M. Hewitt
    Pages 139-148
  11. Anatoly Kiyatkin, Edita Aksamitiene
    Pages 149-161
  12. Oscar Noya, Sandra Losada, Marilyan Toledo, Belkisyolé Alarcón de Noya
    Pages 237-251
  13. Edward P. Trieu, Joanne K. Gross, Ira N. Targoff
    Pages 259-275
  14. Ioannis N. Manoussopoulos, Mina Tsagris
    Pages 277-287
  15. Olga Yeliosof, R. Hal Scofield
    Pages 289-292
  16. Kazuya Machida, Bruce J. Mayer
    Pages 313-329
  17. Jose L. Luque-Garcia, Thomas A. Neubert
    Pages 331-341
  18. Alexander E. Kalyuzhny
    Pages 355-365
  19. Biji T. Kurien, R. Hal Scofield
    Pages 367-384
  20. Neil W. Charter, Joe Horecka, Chin-Yee Loh, Albert Doan, Tom Wehrman, Keith R. Olson
    Pages 395-405
  21. F. Javier Alba, Salvador Bartolomé, Antonio Bermúdez, Joan-Ramon Daban
    Pages 407-416
  22. Anil D’souza, R. Hal Scofield
    Pages 433-440
  23. Claudia Franke, Daniel Gräfe, Holger Bartsch, Michael Bachmann
    Pages 441-449
  24. Holger Bartsch, Claudia Franke, Michael Bachmann
    Pages 451-456
  25. Stan Krajewski
    Pages 463-472
  26. Stan Krajewski, Xianshu Huang, Maryla Krajewska
    Pages 473-481

About this book


Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In Protein Blotting and Detection: Methods and Protocols, expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Innovative and highly practical, Protein Blotting and Detection: Methods and Protocols is an essential, hands-on guide for all investigators who hope to bring these cutting-edge procedures home to their laboratories.


Immunoblotting Membrane proteomics Polyacrylamid-Gelelektrophorese Protein transfer Proteomics SDS-PAGE TLC-Blot Western blotting molecular biology

Editors and affiliations

  • Biji T. Kurien
    • 1
  • R. Hal Scofield
    • 2
  1. 1.Oklahoma Medical Research FoundationOklahoma CityU.S.A.
  2. 2.Oklahoma Medical Research FoundationOklahoma CityU.S.A.

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-73-2
  • Online ISBN 978-1-59745-542-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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