Plant Hormones

Methods and Protocols

  • Sean Cutler
  • Dario Bonetta

Part of the Methods in Molecular Biology book series (MIMB, volume 495)

Table of contents

  1. Front Matter
    Pages i-viii
  2. Danielle Marcos, Thomas Berleth
    Pages 11-20
  3. Masanori Okamoto, Atsushi Hanada, Yuji Kamiya, Shinjiro Yamaguchi, Eiji Nambara
    Pages 53-60
  4. Stefan Kepinski
    Pages 61-80
  5. Ana Caño-Delgado, Zhi-Yong Wang
    Pages 81-88
  6. Fawzi A. Razem, Robert D. Hill
    Pages 89-99
  7. Masayuki Higuchi, Tatsuo Kakimoto, Takeshi Mizuno
    Pages 101-109
  8. Georgy A. Romanov, Sergey N. Lomin
    Pages 111-120
  9. Back Matter
    Pages 145-146

About this book


The last ten years have witnessed a monumental increase in our understanding of plant hormones. A decade ago, scientists were forced to rely on vague ideas about hormone action; today, these have been replaced by detailed molecular models. Given the rapid increase in our understanding of plant hormone biology, this comprehensive review could not have come at a better time. In Plant Hormones: Methods and Protocols, Second Edition, expert researchers explore the most current genetic, biochemical, analytical and chemical biological approaches for understanding plant hormone action, providing a concise overview of methods and reagents needed to dissect plant signalling pathways using chemical genetic methods. Chapters address such topics as genetic methods of analysis using the model system Arabidopsis thaliana, biochemical methods for documenting interactions between hormones and receptors, the use of high-throughput sequencing technologies for microRNA analysis, and analytical approaches for measuring endogenous plant hormone levels, including LC-MS and GC-MS based methods. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Innovative and cutting edge, Plant Hormones: Methods and Protocols, Second Edition is an essential guide for all plant scientists who are facing the complex and challenging questions that will determine the future of this vital field.



454 sequencing technology Arabidopsis thaliana Chemical genetics Expression Liana MicroRNA analysis Plant growth regulators Signal transduction Transport gene expression

Editors and affiliations

  • Sean Cutler
    • 1
  • Dario Bonetta
    • 2
  1. 1.Dept. BotanyUniversity of TorontoTorontoCanada
  2. 2.Dept. BotanyUniversity of TorontoTorontoCanada

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-32-9
  • Online ISBN 978-1-59745-477-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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