Gene Knockout Protocols

Second Edition

  • Wolfgang Wurst
  • Ralf Kühn

Part of the Methods in Molecular Biology book series (MIMB, volume 530)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Gene Modification in ES Cells

    1. Front Matter
      Pages 1-1
    2. Ralf Kühn, Wolfgang Wurst
      Pages 1-12
    3. Song-Choon Lee, Wei Wang, Pentao Liu
      Pages 15-27
    4. Silke De-Zolt, Joachim Altschmied, Patricia Ruiz, Harald von Melchner, Frank Schnütgen
      Pages 29-47
    5. Louise van der Weyden, Charles Shaw-Smith, Allan Bradley
      Pages 49-77
    6. Marieke Aarts, Marleen Dekker, Rob Dekker, Sandra de Vries, Anja van der Wal, Eva Wielders et al.
      Pages 79-99
    7. Christiane Hitz, Patricia Steuber-Buchberger, Sabit Delic, Wolfgang Wurst, Ralf Kühn
      Pages 101-129
    8. Robert Munroe, John Schimenti
      Pages 131-138
  3. Stem Cell Manipulation

    1. Front Matter
      Pages 139-139
    2. Lino Tessarollo, Mary Ellen Palko, Keiko Akagi, Vincenzo Coppola
      Pages 141-164
    3. Eileen Southon, Lino Tessarollo
      Pages 165-185
    4. Heidrun Kern, Branko Zevnik
      Pages 187-204
    5. Eva Wielders, Marleen Dekker, Hein te Riele
      Pages 205-218
    6. Insa S. Schroeder, Cornelia Wiese, Thuy T. Truong, Alexandra Rolletschek, Anna M. Wobus
      Pages 219-250
    7. Sayaka Wakayama, Satoshi Kishigami, Teruhiko Wakayama
      Pages 251-265
  4. Genetically Engineered Mice

    1. Front Matter
      Pages 266-266
    2. Susan W. Reid, Lino Tessarollo
      Pages 269-285
    3. Mika Tanaka, Anna-Katerina Hadjantonakis, Kristina Vintersten, Andras Nagy
      Pages 287-309
    4. Thomas M. DeChiara, William T. Poueymirou, Wojtek Auerbach, David Frendewey, George D. Yancopoulos, David M. Valenzuela
      Pages 311-324
    5. Jan Rodriguez Parkitna, David Engblom, Günther Schütz
      Pages 325-342
    6. Susanne Feil, Nadejda Valtcheva, Robert Feil
      Pages 343-363
    7. Andras Nagy, Lynn Mar, Graham Watts
      Pages 365-378
    8. David A. Largaespada
      Pages 379-390
    9. Alexander Pfeifer, Andreas Hofmann
      Pages 391-405
    10. Susan Marschall, Auke Boersma, Martin Hrabé de Angelis
      Pages 407-420
  5. Phenotype Analysis

    1. Front Matter
      Pages 421-421
    2. Roderick T. Bronson
      Pages 435-461
    3. Valérie Gailus-Durner*, Helmut Fuchs*, Thure Adler, Antonio Aguilar Pimentel, Lore Becker, Ines Bolle et al.
      Pages 463-509
  6. Back Matter
    Pages 511-520

About this book


With the completion of the mouse and human genome sequences, the functional characterization of every mammalian gene and the deciphering of their molecular interaction network has become the next major challenge, and the mouse genome provides a convenient and vital model for these studies. In Gene Knockout Protocols, Second Edition, distinguished contributors with extensive experience in the gene targeting and mouse genetics fields reveal a comprehensive collection of step-by-step laboratory protocols. Emphasizing the many new mutagenesis techniques developed over the last seven years, the volume covers topics such as gene modification in ES cells, stem cell manipulation, the generation of genetically engineered mice, and mutant phenotype analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Fully updated and authoritative, Gene Knockout Protocols, Second Edition provides a unique collection of expert bench protocols certain to be a valuable resource for those just entering the field of gene targeting but also for all genetic researchers today.


DNA ES cells Gene modification Genetically engineered mice Mouse genome Mutagenesis techniques Mutant Phenotype analysis Stem cell manipulation chromosome cryopreservation genes genetics molecular biology

Editors and affiliations

  • Wolfgang Wurst
  • Ralf Kühn

There are no affiliations available

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-26-8
  • Online ISBN 978-1-59745-471-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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