Neutrophil Methods and Protocols

  • Mark T. Quinn
  • Frank R. DeLeo
  • Gary M. Bokoch

Part of the Methods in Molecular Biology™ book series (MIMB, volume 412)

Table of contents

  1. Front Matter
    Pages i-xx
  2. Neutrophils: An Overview

    1. Front Matter
      Pages 1-1
  3. Neutrophil Isolation and Subcellular Fractionation

    1. Front Matter
      Pages 13-13
    2. William M. Nauseef
      Pages 15-20
    3. Daniel W. Siemsen, Igor A. Schepetkin, Liliya N. Kirpotina, Benfang Lei, Mark T. Quinn
      Pages 21-34
  4. Biochemistry, Biology, and Signal Transduction of Neutrophils

    1. Front Matter
      Pages 57-57
    2. Ulla G. Knaus, Alison Bamberg, Gary M. Bokoch
      Pages 59-67
    3. Susan Sergeant, Linda C. McPhail
      Pages 69-83
    4. Jamel El-Benna, Pham My-Chan Dang
      Pages 85-96
    5. Martine Torres
      Pages 97-113
    6. Tieming Zhao, Gary M. Bokoch
      Pages 115-123
    7. Esther J. Hillson, Sharon Dewitt, Maurice B. Hallett
      Pages 125-137
    8. Deri Morgan, Thomas E. DeCoursey
      Pages 139-175
    9. Emma L. Taylor, Adriano G. Rossi, Ian Dransfield, Simon P. Hart
      Pages 177-200
  5. Neutrophil Adhesion and Chemotaxis

    1. Front Matter
      Pages 201-201
    2. Ulrich Y. Schaff, Melissa R. Sarantos, Harold Ting, Scott I. Simon
      Pages 203-210
    3. Mary A. Lokuta, Paul A. Nuzzi, Anna Huttenlocher
      Pages 211-229
    4. Mark A. Jutila, Bruce Walcheck, Robert Bargatze, Aiyappa Palecanda
      Pages 239-256
    5. Nancy A. Louis, Eric Campbell, Sean P. Colgan
      Pages 257-270
  6. Neutrophil Phagocytosis and Bactericidal Activity

    1. Front Matter
      Pages 271-271
    2. Benjamin E. Steinberg, Sergio Grinstein
      Pages 289-300
    3. Per Lönnbro, Pontus Nordenfelt, Hans Tapper
      Pages 301-318
    4. Jessie N. Green, Christine C. Winterbourn, Mark B. Hampton
      Pages 319-332
  7. Nadph Oxidase and Production of Reactive Oxygen Species

    1. Front Matter
      Pages 347-347
    2. Claes Dahlgren, Anna Karlsson, Johan Bylund
      Pages 349-363
    3. Thomas L. Leto, Mark C. Lavigne, Neda Homoyounpour, Kristen Lekstrom, Gilda Linton, Harry L. Malech et al.
      Pages 365-383
    4. Shahar Molshanski-Mor, Ariel Mizrahi, Yelena Ugolev, Iris Dahan, Yevgeny Berdichevsky, Edgar Pick
      Pages 385-428
  8. Analysis of Neutrophil Gene Expression and Transcription Factors

    1. Front Matter
      Pages 439-439
    2. Scott D. Kobayashi, Dan E. Sturdevant, Frank R. DeLeo
      Pages 441-453
    3. Patrick P. McDonald, Richard D. Ye
      Pages 473-486
  9. Neutrophil Defects and Diagnosis

    1. Front Matter
      Pages 487-487
    2. Mary C. Dinauer
      Pages 489-504
    3. Houda Zghal Elloumi, Steven M. Holland
      Pages 505-523
    4. William M. Nauseef
      Pages 525-530
  10. Back Matter
    Pages 531-537

About this book


Neutrophil Methods and Protocols provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Parts 1 & 2 provide an overview of neutrophils the methods most commonly used for isolating them from humans and other animal species. Part 3 details methods for investigating adhesion, chemotaxis, phagocytosis and bacterial activity. Part 4 presents protocols measuring gene expression in neutrophils, and Part 5 concludes with assays for diagnosing the most common neutrophil disorders. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.


Activation Endoplasmatisches Reticulum Immune System PCR bactericidal activity biochemistry gene expression genes microscopy neutrophils signal transduction transcription

Editors and affiliations

  • Mark T. Quinn
    • 1
  • Frank R. DeLeo
    • 2
  • Gary M. Bokoch
    • 3
  1. 1.Deparment of Veterinary Molecular BiologyMontana State UniversityBozeman
  2. 2.Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious DiseasesNational Institutes of HealthHamilton
  3. 3.Departments of Immunology and Cell BiologyThe Scripps InstituteLa Jolla

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-788-4
  • Online ISBN 978-1-59745-467-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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