Epitope Mapping Protocols

Second Edition

  • Mike Schutkowski
  • Ulrich Reineke

Part of the Methods in Molecular Biology™ book series (MIMB, volume 524)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. B-cell Epitope Mapping

    1. Front Matter
      Pages 1-1
    2. Marc H.V. Van Regenmortel
      Pages 3-20
  3. Whole Antigen Methods

    1. Front Matter
      Pages 22-22
    2. Maria R. Mazzoni, Francesca Porchia, Heidi E. Hamm
      Pages 77-86
    3. Suraj Dhungana, Jason G. Williams, Michael B. Fessler, Kenneth B. Tomer
      Pages 87-101
    4. Howard M. Reisner, Roger L. Lundblad
      Pages 103-117
    5. Suraj Dhungana, Michael B. Fessler, Kenneth B. Tomer
      Pages 119-134
  4. Peptide Library Approaches

    1. Front Matter
      Pages 136-136
    2. Michael W. Heuzenroeder, Mary D. Barton, Thiru Vanniasinkam, Tongted Phumoonna
      Pages 137-144
    3. Ulrich Reineke, Robert Sabat
      Pages 145-167
    4. Johannes Zerweck, Antonia Masch, Mike Schutkowski
      Pages 169-180
    5. Volker Böttger, Angelika Böttger
      Pages 181-201
    6. Dawn R. Mattoon, Barry Schweitzer
      Pages 213-223
  5. Profiling of Antibody Signatures in Biological Fluids.

    1. Front Matter
      Pages 236-236
    2. Juliane Bongartz, Nicole Bruni, Michal Or-Guil
      Pages 237-246
    3. Peter Lorenz, Michael Kreutzer, Johannes Zerweck, Mike Schutkowski, Hans-Jürgen Thiesen
      Pages 247-258
    4. Jing Lin, Ludmilla Bardina, Wayne G. Shreffler
      Pages 259-272
    5. Lina Cekaite, Eiving Hovig, Mouldy Sioud
      Pages 273-285
  6. Antigen Expressed from Recombinant DNA

    1. Front Matter
      Pages 288-288
    2. Tsutomu Mikawa, Masayuki lkeda, Takehiko Shibata
      Pages 305-313
  7. B-Cell Epitope Prediction

    1. Front Matter
      Pages 334-334
    2. Ulf Reimer
      Pages 335-344
  8. T-Cell Epitope Mapping

    1. Front Matter
      Pages 346-346
    2. Sine Reker Hadrup, Mireille Toebes, Boris Rodenko, Arnold H. Bakker, David A. Egan, Huib Ovaa et al.
      Pages 383-405
    3. Alejandra Nacarino Martinez, Stefan Tenzer, Hansjörg Schild
      Pages 407-415
    4. Raija K.S. Ahmed, Markus J. Maeurer
      Pages 427-438
    5. Markus Wulf, Petra Hoehn, Peter Trinder
      Pages 439-446
  9. Back Matter
    Pages 447-456

About this book


Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear.  In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping.


Antibody signatures Antigen Antigen processing B-cells Biomarker discovery DNA Microarray PCR Peptide library Protein domains T-cell receptors Termination autoimmune disease molecular biology

Editors and affiliations

  • Mike Schutkowski
  • Ulrich Reineke

There are no affiliations available

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-17-6
  • Online ISBN 978-1-59745-450-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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