Extracellular Matrix Protocols

Second Edition

  • Sharona Even-Ram
  • Vira Artym

Part of the Methods in Molecular Biology book series (MIMB, volume 522)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Molecular Biology

    1. Front Matter
      Pages 1-1
    2. Vitali Alexeev, Olga Igoucheva
      Pages 3-14
    3. Mara Brancaccio, Emila Turco, Emilio Hirsch
      Pages 15-50
    4. David Staunton, Christopher J. Millard, A. Radu Aricescu, Iain D Campbell
      Pages 73-99
    5. Nicholas C. Avery, Trevor J. Sims, Allen J. Bailey
      Pages 103-121
  3. Biochemical and biophysical analysis

    1. Front Matter
      Pages 1-1
    2. Nigel W. Hodson, Cay M. Kielty, Michael J. Sherratt
      Pages 123-141
    3. Gradimir N. Misevic, Yannis Karamanos, Nikola J. Misevic
      Pages 143-150
    4. Michael J. Sherratt, Helen K. Graham, Cay M. Kielty, David F. Holmes
      Pages 151-161
    5. Gradimir N. Misevic, Bernard Rasser, Vic Norris, Cédric Dérue, David Gibouin, Fabrice Lefebvre et al.
      Pages 163-173
    6. Michael J. Sherratt, Roger S. Meadows, Helen K. Graham, Cay M. Kielty, David F. Holmes
      Pages 175-181
    7. Emanuele Ostuni, George M. Whitesides, Donald E. Ingber, Christopher S. Chen
      Pages 183-194
    8. Martin J. Humphries
      Pages 203-210
  4. Cell biology assays

    1. Front Matter
      Pages 1-1
    2. Vira V. Artym, Kenneth M. Yamada, Susette C. Mueller
      Pages 211-219
    3. Paola Spessotto, Katia Lacrima, Pier Andrea Nicolosi, Eliana Pivetta, Martina Scapolan, Roberto Perris
      Pages 221-250
    4. Patricia Simon-Assmann, Anne-Laure Bolcato-Bellemin, Annick Klein, Michèle Kedinger
      Pages 309-318
  5. Organ models

  6. Tissue Engineering

    1. Front Matter
      Pages 1-1
    2. Iossif A. Strehin, Jennifer H. Elisseeff
      Pages 349-362
  7. Back Matter
    Pages 371-375

About this book


The study of the extracellular matrix has significantly expanded over the past decade. Although thoroughly researched, the complexity and the dynamic nature of the matrix are still under extensive investigation, yielding a growing number of methods of analysis. In Extracellular Matrix Protocols: Second Edition, expert researchers explore this developing field and provide a number of techniques which can be reproduced in most cell biology labs. This edition contains methods that represent cutting edge unique technologies which are well worth exposure to the broad field and may encourage collaboration with expert labs. Chapters include information on molecular biology techniques to study gene expression, biophysical and biochemical methods for the analysis of structure and composition, and cell biology methods for assessment of cell behavior, matrix assembly and tissue engineering applications. Composed in the highly successful Methods in Molecular Biology(tm) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Extracellular Matrix Protocols: Second Edition investigates the diverse roles of the extracellular matrix, providing important tools for all researchers in the ECM community.


Cell behavior Gene expression Matrix assembly Termination Tissue engineering electron microscopy genes molecular biology

Editors and affiliations

  • Sharona Even-Ram
    • 1
  • Vira Artym
    • 2
  1. 1.Gene Therapy Inst.Hadassah Univ. HospitalJerusalemIsrael
  2. 2.Lombardi Comprehensive Cancer Ctr.Georgetown UniversityWashingtonU.S.A.

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-984-0
  • Online ISBN 978-1-59745-413-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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