Animal Cell Biotechnology

Methods and Protocols

  • Ralf Pörtner

Part of the Methods in Biotechnology book series (MIBT, volume 24)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Basic Techniques for Establishing Production Cell Lines

    1. Front Matter
      Pages 1-1
    2. David A. Fluri, Martin Fussenegger
      Pages 3-21
    3. Christophe Delenda, Miguel Chillon, Anne-Marie Douar, Otto-Wilhelm Merten
      Pages 23-91
    4. Alexandra Dorn-Beineke, Stefanie Nittka, Michael Neumaier
      Pages 93-121
    5. Glyn N. Stacey, Ed Byrne, J. Ross Hawkins
      Pages 123-145
  3. Basic Cultivation Techniques

    1. Front Matter
      Pages 147-147
    2. Gerald Blüml
      Pages 149-178
    3. Holger Hübner
      Pages 179-191
    4. Martin Jordan, Nigel Jenkins
      Pages 193-202
  4. Cell Characterization and Analysis

    1. Front Matter
      Pages 203-203
    2. Michael Butler, Maureen Spearman
      Pages 205-222
    3. Silvia Carroll, Mariam Naeiri, Mohamed Al-Rubeai
      Pages 223-237
    4. Adiba Ishaque, Mohamed Al-Rubeai
      Pages 285-299
    5. Chetan T. Goudar, Richard Biener, James M. Piret, Konstantin B. Konstantinov
      Pages 301-317
  5. Special Cultivation Techniques

    1. Front Matter
      Pages 319-319
    2. John M. Davis
      Pages 337-352
    3. Ralf Pörtner, Oscar B. J. Platas Barradas
      Pages 353-369
    4. Dirk E. Martens, Evert Jan van den End
      Pages 371-393
  6. Downstream Techniques

    1. Front Matter
      Pages 395-395
    2. Peter Czermak, Dirk Nehring, Ranil Wickramasinghe
      Pages 397-420
  7. Special Applications

    1. Front Matter
      Pages 455-455
    2. Yvonne Genzel, Udo Reichl
      Pages 457-473
    3. Pedro E. Cruz, Marlene Carmo, Teresa Rodrigues, Paula Alves
      Pages 475-487
  8. Back Matter
    Pages 509-512

About this book


Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation.

Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors.

Animal Cell Biotechnology: Methods and Protocols, Second Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.


DNA Downstream Processing Expression biotechnology cell lines gene therapy genes mammalian cell lines metabolism physiology

Editors and affiliations

  • Ralf Pörtner
    • 1
  1. 1.Institute for Bioprocess and Biochemical EngineeringHamburg University of TechnologyHamburgGermany

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-660-3
  • Online ISBN 978-1-59745-399-8
  • Series Print ISSN 1940-6061
  • Series Online ISSN 1940-607X
  • Buy this book on publisher's site
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