Methods and Protocols

  • Samir S. Ayoub
  • Roderick J. Flower
  • Michael P. Seed

Part of the Methods in Molecular Biology book series (MIMB, volume 644)

Table of contents

  1. Front Matter
    Pages i-x
  2. In Vitro Protocols for Studying Expression and Activity of Cyclooxygenase Enzymes and Characterisation of Their Inhibition Patterns

  3. Extraction and Measurement of Prostanoids and Isoprostanes

    1. Front Matter
      Pages 146-146
    2. Lorenzo Polenzani, Samir S. Ayoub
      Pages 153-164
    3. Stefania Tacconelli, Marta L. Capone, Paola Patrignani
      Pages 165-178
  4. In Vivo Models to Study Involvement of Cyclooxygenase Products in Health and Disease

  5. Back Matter
    Pages 217-218

About this book


Since the discovery of the pharmacological and toxicological importance of inhibiting the cyclooxygenase (COX) enzymes by non-steroidal anti-inflammatory drugs (NSAIDs), much research has gone into the development of methods to study the biological functions of COX-1 and COX-2.  In Cyclooxygenases: Methods and Protocols, experts and pioneers in the field present the most up-to-date in vitro and in vivo techniques routinely used in COX research.  The volume delves into essential topics such as the purification, cloning, and expression of COX enzymes as well as in vitro assays aimed at determining the inhibitory potency of drugs on COX-1 and COX-2 activities, with chapters describing protocols used for the extraction and measurement of the prostanoids. This volume also describes in vivo disease models used to study the roles of COX-1 and COX-2 in gastrointestinal injury, inflammation, and pain.  As a book in the highly successful Methods in Molecular Biology™ series, the protocols chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cyclooxygenases: Methods and Protocols serves as an indispensable tool for all scientists seeking the treatment of inflammation, pain, fever, and other harmful conditions.


COX-1 and COX-2 Cyclooxygenase enzymes Disease processes Expression In vivo models Inhibition patterns Lipid NSAIDs Non-steroidal anti-inflammatory drugs Oxidation Prostanoids and isoprostanes Selective inhibitors cloning enzymes

Editors and affiliations

  • Samir S. Ayoub
    • 1
  • Roderick J. Flower
    • 2
  • Michael P. Seed
    • 3
  1. 1.Queen Mary's School of Med. & Dentistry, William Harvey Research Inst.Barts & The LondonLondonUnited Kingdom
  2. 2.Queen Mary's School of Med. & Dentistry, William Harvey Research Inst.Barts & The LondonLondonUnited Kingdom
  3. 3.Queen Mary's School of Med. & Dentistry, William Harvey Research Inst.Barts & The LondonLondonUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-953-6
  • Online ISBN 978-1-59745-364-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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