Cryopreservation and Freeze-Drying Protocols

  • John G. Day
  • Glyn N. Stacey

Part of the Methods in Molecular Biology™ book series (MIMB, volume 368)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Gerald Adams
    Pages 15-38
  3. David E. Pegg
    Pages 39-57
  4. Paul Matejtschuk
    Pages 59-72
  5. Chris Bond
    Pages 99-107
  6. Chris Bond
    Pages 109-117
  7. C. Shu-hui Tan, Cor W. van Ingen, Joost A. Stalpers
    Pages 119-125
  8. Brian W. W. Grout
    Pages 153-161
  9. Erica E. Benson, Keith Harding, Jason W. Johnston
    Pages 163-183
  10. Hugh W. Pritchard
    Pages 185-201
  11. Eugeny Kopeika, Julia Kopeika, Tiantian Zhang
    Pages 203-217
  12. Graham J. Wishart
    Pages 219-225
  13. Christopher B. Morris
    Pages 227-236
  14. Suzanne M. Watt, Eric Austin, Sue Armitage
    Pages 237-259
  15. Charles J. Hunt, Paula M. Timmons
    Pages 261-270
  16. Glyn N. Stacey, Stuart Dowall
    Pages 271-281
  17. Mark R. Curry
    Pages 303-311
  18. Sharon J. Paynter, Barry J. Fuller
    Pages 313-324
  19. Barry J. Fuller, Sharon J. Paynter
    Pages 325-339
  20. Back Matter
    Pages 341-347

About this book


Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995.

This volume begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Detailed descriptions of materials required and stepwise protocols are presented for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources.


biological materials biological resource centers biological resource centres fish sperm human cell lines human embryonic stem cell lines lyophilization mammalian semen multicellular structures prokaryotes red blood cells reproducible techniques shelf-freeze-drier vacuum-drying yeast cultures

Editors and affiliations

  • John G. Day
    • 1
  • Glyn N. Stacey
    • 2
  1. 1.Culture Collection of Algae and ProtozoaScottish Association for Marine Science, Dunstaffnage Marine LaboratoryDunbeg, ArgyllUK
  2. 2.Division of Cell Biology and ImagingNational Institute for Biological Standards and ControlPotters Bar, HertsUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-377-0
  • Online ISBN 978-1-59745-362-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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