Quantitative Proteomics by Mass Spectrometry

  • Salvatore Sechi

Part of the Methods in Molecular Biology book series (MIMB, volume 359)

Table of contents

  1. Front Matter
    Pages i-x
  2. Illarion V. Turko, Salvatore Sechi
    Pages 1-16
  3. Scott A. Gerber, Arminja N. Kettenbach, John Rush, Steven P. Gygi
    Pages 71-86
  4. Tao Liu, Wei-Jun Qian, David G. Camp II, Richard D. Smith
    Pages 107-124
  5. Catherine Fenselau, Xudong Yao
    Pages 135-142
  6. František Tureček, C. Ronald Scott, Michael H. Gelb
    Pages 143-157
  7. Yu Lu, Patricia Bottari, Ruedi Aebersold, František Tureček, Michael H. Gelb
    Pages 159-176
  8. Yi Zhang, Alejandro Wolf-Yadlin, Forest M. White
    Pages 203-212
  9. Back Matter
    Pages 213-218

About this book


Quantitative Proteomics by Mass Spectrometry, from the Methods in Molecular Biology™ series, is a compendium of cutting-edge protocols for quantitative proteomics, and presents the most significant methods used in the field today. The focus on mass spectrometry (MS) is integral, as MS has, and will continue to be, an essential tool in proteomics for studying complex biological systems and human diseases. This volume, written and compiled by leading quantitative proteomic experts, is an indispensable resource in the search for novel biomarkers.

Quantitative Proteomics by Mass Spectrometry presents several innovative MS quantitative procedures, including a variety of methods for introducing isotopic labels and quantifying post-translational modifications. Some of these methods include growing an organism in isotope-enriched media, performing trypsin proteolysis in the presence of 18O-water, reacting protein samples with isotopically labeled reagents, quantifying relative amount of proteins without the use of any isotopic labels. Attention is also given to state-of-the-art techniques for the characterization of the phosphoproteome and tandem MS for detection of inborn errors of metabolism. Specifically, the procedure for determinations of enzymatic activity could be used for large-scale screening of newborns. The protocols in this volume expand both the breadth and depth of readily available methods for quantitative proteomic researchers using MS..


Amino acid Proteomics Termination Translation metabolism molecular biology post-translational modification protein complexes proteins

Editors and affiliations

  • Salvatore Sechi
    • 1
  1. 1.National Institute of Diabetes and Digestive and Kidney DiseasesNational Institutes of HealthBethesda

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-571-2
  • Online ISBN 978-1-59745-255-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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