Methods and Protocols

  • Henrik Nielsen

Part of the Methods in Molecular Biology book series (MIMB, volume 703)

Table of contents

  1. Front Matter
    Pages i-xv
  2. RNA Methods

    1. Front Matter
      Pages 1-1
    2. Henrik Nielsen
      Pages 3-14
    3. Henrik Nielsen
      Pages 15-28
    4. Bertrand Beckert, Benoît Masquida
      Pages 29-41
    5. Nana Jacobsen, Jens Eriksen, Peter Stein Nielsen
      Pages 43-51
    6. Elfar Torarinsson
      Pages 53-65
    7. Ajish D. George, Scott A. Tenenbaum
      Pages 67-86
    8. Knud Josefsen, Henrik Nielsen
      Pages 87-105
    9. Oladapo Yeku, Michael A. Frohman
      Pages 107-122
    10. Hedda A. Meijer, Cornelia H. de Moor
      Pages 123-135
    11. Zhaiyi Zhang, Stefan Stamm
      Pages 137-160
    12. Martin Lützelberger, Jørgen Kjems
      Pages 161-171
    13. Jørn Remi Henriksen, Jochen Buechner, Cecilie Løkke, Trond Flægstad, Christer Einvik
      Pages 189-204
    14. Knud Josefsen, Ying C. Lee
      Pages 205-217
    15. Joel Nelson, Oleg Denisenko, Karol Bomsztyk
      Pages 219-234
  3. RNP Methods

    1. Front Matter
      Pages 235-235
    2. Scott A. Tenenbaum, Jan Christiansen, Henrik Nielsen
      Pages 237-245
    3. Ritu Jain, Tiffany Devine, Ajish D. George, Sridar V. Chittur, Timothy E. Baroni, Luiz O. Penalva et al.
      Pages 247-263
    4. Lars Jønson, Finn Cilius Nielsen, Jan Christiansen
      Pages 265-273
    5. Tomáš Mašek, Leoš Valášek, Martin Pospíšek
      Pages 293-309
    6. Morten Lindow
      Pages 311-317
    7. Henrik Nielsen
      Pages 319-325
  4. Back Matter
    Pages 327-329

About this book


Recent insight into the transcripts generated from the mammalian genome (i.e. the transcriptome) has revealed that transcription is a far more complex phenomenon than previously thought. In RNA: Methods and Protocols, expert researchers provide the procedures and methods used to describe the structure of messenger RNAs and non-coding RNAs that are transcribed by RNA polymerase II as the immediate gene products in mammalian cells. Focused on the structure of the RNA products of “gene X” and the mapping of proteins associated with these RNAs, the volume presents appropriate information for non-specialists in RNA biology. Written in the highly successful Methods in Molecular Biology™ series format, many chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, RNA: Methods and Protocols views the transcriptional landscape with an appreciation for the role that proteins play in the processing and interpretation of genetic information in an attempt to further our crucial knowledge of the many products and sophisticated regulatory networks that result from it.


Bioinformatics Mammalian genome Messenger RNAs Microarray analysis Non-coding RNAs Protein mapping RNA polymerase II RNP methods Transcriptional landscape Transcriptome miRNA profiling

Editors and affiliations

  • Henrik Nielsen
    • 1
  1. 1.The Panum Institute, Dept. of Cellular & Molecular MedicineUniversity of CopenhagenCopenhagenDenmark

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-913-0
  • Online ISBN 978-1-59745-248-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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