Methods and Protocols

  • Wolfram Weckwerth

Part of the Methods in Molecular Biology™ book series (MIMB, volume 358)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Metabolome Analysis Using Gas Chromatography Coupled to Mass Spectrometry

    1. Front Matter
      Pages 1-1
    2. Oliver Fiehn, Tobias Kind
      Pages 3-17
    3. Yury M. Tikunov, Francel W. A. Verstappen, Robert D. Hall
      Pages 39-53
  3. Metabolomics: Data Integration and Data Mining

    1. Front Matter
      Pages 17-17
    2. Katja Morgenthal, Stefanie Wienkoop, Florian Wolschin, Wolfram Weckwerth
      Pages 57-75
    3. Ewa Urbanczyk-Wochniak, Lothar Willmitzer, Alisdair R. Fernie
      Pages 77-85
    4. Matthias Scholz, Joachim Selbig
      Pages 87-104
    5. Ralf Steuer, Katja Morgenthal, Wolfram Weckwerth, Joachim Selbig
      Pages 105-126
  4. Capillary Electrophoresis Coupled to Mass Spectrometry

    1. Front Matter
      Pages 37-37
  5. Liquid Chromatography Coupled to Mass Spectrometry for Metabolomics and Structural Elucidation

    1. Front Matter
      Pages 55-55
    2. Vladimir V. Tolstikov, Oliver Fiehn, Nobuo Tanaka
      Pages 141-155
  6. Electrochemical Detection of Metabolites

    1. Front Matter
      Pages 75-75
    2. Bruce S. Kristal, Yevgeniya I. Shurubor, Rima Kaddurah-Daouk, Wayne R. Matson
      Pages 159-174
  7. Metabolic Fluxes

    1. Front Matter
      Pages 175-175
    2. Stefan Schuster, Axel von Kamp, Mikhail Pachkov
      Pages 199-226
  8. NMR Metabolome Analysis

  9. Back Matter
    Pages 287-293

About this book


Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.

Part I of this volume focuses on GC-MS techniques; specifically, attention is given to metabolite profiling in blood plasma, construction and application of mass spectral and retention time index libraries, and metabolomic profiling of natural volatiles. In Part II, emphasis shifts to data integration and data mining techniques, and specific protocols discuss improving pattern recognition and biomarker selection for systems level approaches, and visualization and analysis of molecular and metabolomic data. Parts III, IV, and V examine the coupling of capillary electrophoresis and mass spectrometry, classical reversed phase liquid chromatography-mass spectrometry, and electrochemical detection of metabolites using HPLC separations coupled with coulometric electrode array detectors. In Part VI, protocols for the determination of metabolic flux rates are presented and a theoretical framework for metabolic pathway network analysis is provided. The volume concludes with an examination of cutting-edge NMR protocols.


Chromatography Mass Spectrometry Termination data mining metabolism

Editors and affiliations

  • Wolfram Weckwerth
    • 1
  1. 1.Department of Metabolic NetworksMax Planck Institute of Molecular Plant PhysiologyPotsdam-GolmGermany

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-561-3
  • Online ISBN 978-1-59745-244-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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