Methods and Protocols

  • Laura E. Nagy

Part of the Methods in Molecular Biology™ book series (MIMB, volume 447)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Animal Models of Acute and Chronic Ethanol Exposure

    1. Timothy P. Plackett, Elizabeth J. Kovacs
      Pages 3-9
    2. George L. Tipoe, Emily C. Liong, Tung-Ming Leung, Amin A. Nanji
      Pages 11-31
    3. Hide Tsukamoto, Hasmik Mkrtchyan, Alla Dynnyk
      Pages 33-48
    4. Ruth A. Coleman, Betty M. Young, Lucas E. Turner, Robert T. Cook
      Pages 49-59
  3. Animal Models of Ethanol Exposure During Development

    1. Robert L. Tanguay, Mark J. Reimers
      Pages 63-74
    2. Susan M. Smith
      Pages 75-84
    3. Hector D. Dominguez, Jennifer D. Thomas
      Pages 85-100
    4. Sandra J. Kelly, Charles R. Lawrence
      Pages 101-110
  4. Cell Culture Approaches to Studying Ethanol Exposure

    1. Amanda Lindke, Barbara Tremper-Wells, Michael W. Miller
      Pages 125-136
    2. Rajesh C. Miranda, Daniel R. Santillano, Cynthia Camarillo, Douglas Dohrman
      Pages 151-168
  5. Regulation of Specific Organ Systems in Response to Ethanol

    1. Megan R. McMullen, Michele T. Pritchard, Laura E. Nagy
      Pages 199-212
    2. Kevin L. Legge, Annette J. Schlueter
      Pages 213-233
    3. Betty M. Young, Susan Wiechert, Ruth A. Coleman, Prajwal Gurung, Robert T. Cook
      Pages 277-294
    4. Shilpi Verma, Carla-Maria A. Alexander, Michael J. Carlson, Lorraine T. Tygrett, Thomas J. Waldschmidt
      Pages 295-323
    5. Urszula T. Iwaniec, Thomas J. Wronski, Russell T. Turner
      Pages 325-341
    6. Becky M. Sebastian, Li Kang, Xiaocong Chen, Laura E. Nagy
      Pages 357-366
  6. Proteomic and Genomic Approaches to Study Response to Ethanol

    1. Shannon M. Bailey, Kelly K. Andringa, Aimee Landar, Victor M. Darley-Usmar
      Pages 369-380
    2. Robnet T. Kerns, Michael F. Miles
      Pages 395-410
  7. Back Matter
    Pages 411-414

About this book


Many unexplored complex cellular and organismal adaptations occur in response to the stress of alcohol exposure, and its contribution to the development of chronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population. In Alcohol: Methods and Protocols, the pleiotropic effects of ethanol in animal and cell culture models are rigorously examined through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics. As part of the successful Methods in Molecular Biology™ series, each chapter provides step-by-step laboratory protocols, lists of the necessary materials, and a Notes section with tips on troubleshooting and avoiding known pitfalls.

Comprehensive and highly specific, Alcohol: Methods and Protocols strives to further spur investigators to delve into the complex and fascinating processes of the adaptive and maladaptive responses humans have developed to the consumption of alcohol.


Adaptive and maladaptive response Antigen Cell and tissue development Chronic ethanol exposure Ethanol Excessive alcohol consumption Microarray Pathophysiology Regulation bone cells gene expression genes tissue

Editors and affiliations

  • Laura E. Nagy
    • 1
  1. 1.Departments of Gastroenterology and Pathobiology Cleveland Clinic and Department of NutritionCase Western Reserve UniversityClevelandUSA

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-906-2
  • Online ISBN 978-1-59745-242-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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