Chromosomal Mutagenesis

  • Gregory D. Davis
  • Kevin J. Kayser

Part of the Methods in Molecular Biology book series (MIMB, volume 435)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Naoko Yamane-Ohnuki, Kazuya Yamano, Mitsuo Satoh
    Pages 1-16
  3. Jean-Pierre Cabaniols, Frédéric Pâques
    Pages 31-45
  4. Dana Carroll, Kelly J. Beumer, J. Jason Morton, Ana Bozas, Jonathan K. Trautman
    Pages 63-77
  5. Knut Woltjen, Kenichi Ito, Teruhisa Tsuzuki, Derrick E. Rancourt
    Pages 79-94
  6. David A. Largaespada, Lara S. Collier
    Pages 95-108
  7. Junji Takeda, Zsuzsanna Izsvák, Zoltán Ivics
    Pages 109-125
  8. Thomas Floss, Frank Schnütgen
    Pages 127-138
  9. Sareina Chiung-Yuan Wu, Kommineni J. Maragathavally, Craig J. Coates, Joseph M. Kaminski
    Pages 139-151
  10. Troy L. Brady, Clarice L. Schmidt, Daniel F. Voytas
    Pages 153-163
  11. Annahita Keravala, Michele P. Calos
    Pages 165-173
  12. Erica B. Schleifman, Joanna Y. Chin, Peter M. Glazer
    Pages 175-190
  13. Julia C. van Kessel, Graham F. Hatfull
    Pages 203-215
  14. Back Matter
    Pages 229-235

About this book


Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation.  In Chromosomal Mutagenesis, a team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond model organisms.  Following the format of the highly successful Methods in Molecular Biology™ format, each chapter offers step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Chomosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types.


DNA Eukaryotic organisms Evolution Gene disruption Gene knockouts Gene therapy Homologous recombination Mutation Prokaryotic organisms chromosome genes molecular biology recombination

Editors and affiliations

  • Gregory D. Davis
    • 1
  • Kevin J. Kayser
    • 2
  1. 1.Sigma-Aldrich BiotechnologySigma-Aldrich CorporationSt. Louis
  2. 2.SAFC BiosciencesSigma-Aldrich CorporationSt. Louis

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-899-7
  • Online ISBN 978-1-59745-232-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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