The Protein Protocols Handbook

  • John M. Walker

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xxxv
  2. Quantitation of Proteins

    1. Alastair Aitken, Michèle P. Learmonth
      Pages 3-6
    2. Jakob H. Waterborg
      Pages 7-10
    3. Nicholas J. Kruger
      Pages 17-24
    4. Robert E. Akins, Rocky S. Tuan
      Pages 25-34
    5. Scott A. Boerner, Crescent R. Isham, Yean Kit Lee, Jennifer D. Tibodeau, Scott H. Kaufmann, Keith C. Bible
      Pages 35-45
    6. Alastair Aitken, Michèle P. Learmonth
      Pages 47-50
    7. Douglas D. Root, Kuan Wang
      Pages 51-54
    8. Thomas D. Friedrich, F. Andrew Ray, Ralph L. Smith, John M. Lehman
      Pages 55-61
    9. Thomas D. Friedrich, Ralph L. Smith, John M. Lehman
      Pages 63-69
  3. Electrophoresis of Proteins and Peptides and Dectection in Gels

    1. Sebastien Christian Carpentier, Rony Swennen, Bart Panis
      Pages 109-119
    2. Brian B. Vandahl, Gunna Christiansen, Svend Birkelund
      Pages 121-130
    3. Anthony G. Sullivan, Henry Brzeski, Jeban Ganesalingam, Manuel Mayr
      Pages 131-138
    4. Lynn A. Echan, David W. Speicher
      Pages 139-153
    5. Joakim Norbeck
      Pages 155-158
    6. Jens Schindler, Hans Gerd Nothwang
      Pages 159-164
    7. Hans Gerd Nothwang, Isabelle Guillemin, Jens Schindler
      Pages 165-170
    8. Elke A. Dian, Joachim Rassow, Christian Motz
      Pages 201-210
    9. Sylvie Luche, Mireille Chevallet, Cécile Lelong, Thierry Rabilloud
      Pages 211-219
    10. Slobodan Poznanović, Wojciech Wozny, Helmut Zengerling, Gerhard P. Schwall, Michael A. Cahill
      Pages 269-277
    11. Masamichi Oh-Ishi, Tadakazu Maeda
      Pages 299-304
    12. Elisabetta Gianazza
      Pages 305-322
    13. Barbara A. Fogarty, Nathan A. Lacher, Susan M. Lunte
      Pages 329-359
    14. Andrea W. Chow, Bahram Fathollahi
      Pages 361-378
    15. David B. Friedman, Kathryn S. Lilley
      Pages 379-408
    16. Peter F. Lemkin, Gregory C. Thornwall, Jai A. Evans
      Pages 409-441
    17. Wojciech Wozny, Gerhard P. Schwall, Chaturvedula S. Sastri, Slobodan Poznanović, Werner Stegmann, Christian Hunzinger et al.
      Pages 449-478
    18. Bryan John Smith
      Pages 479-486
    19. Joan-Ramon Daban, Salvador Bartolomé, Antonio Bermúdez, F. Javier Alba
      Pages 497-504
    20. Carlos Fernandez-Patron
      Pages 505-513
    21. Wei-Tao Cong, Sun-Young Hwang, Li-Tai Jin, Jung-Kap Choi
      Pages 515-520

About this book


Since the publication of the bestselling second edition of John Walker's widely acclaimed Protein Protocols Handbook, there have been continual methodological developments in the field of protein chemistry.  This greatly enhanced third edition introduces 57 critically important new chapters, as well as significantly updating the previous edition's tried-and-true methods.  Although the timely new chapters are spread throughout all of the book, the vital section on post-translational modifications has been expanded most to reflect the increasing importance of these modifications in the understanding of protein function. 

Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances.  The expert authors of each chapter have demonstrated a hands-on mastery of the methods described, fine-tuned here for optimal productivity.

Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Third Edition is today's indispensable benchtop manual and guide, not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armametarium of techniques in the urgent search for rapid and robust results.


Antibodies Electrophoresis Post-translational modifications Protein blotting Protein function Translation proteins

Editors and affiliations

  • John M. Walker
    • 1
  1. 1.School of Life SciencesUniversity of HertfordshireHatfieldUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-474-6
  • Online ISBN 978-1-59745-198-7
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site
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