Design and Application

  • Sailen Barik

Part of the Methods in Molecular Biology™ book series (MIMB, volume 442)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Application of RNAi in Diverse Organisms

    1. Front Matter
      Pages 1-1
    2. Mohammed Amarzguioui, John J. Rossi
      Pages 3-10
    3. Keita Miyoshi, Hiroshi Uejima, Tomoko Nagami-Okada, Haruhiko Siomi, Mikiko C. Siomi
      Pages 29-43
    4. Emily Anderson, Queta Boese, Anastasia Khvorova, Jon Karpilow
      Pages 45-63
  3. Application of RNAi in Diverse Organisms

    1. Front Matter
      Pages 65-65
    2. Doreen E. Wesche-Soldato, Joanne Lomas-Neira, Mario Perl, Chun-Shiang Chung, Alfred Ayala
      Pages 67-73
    3. Vira Bitko, Sailen Barik
      Pages 75-82
    4. Vivian Bellofatto, Jennifer B. Palenchar
      Pages 83-94
    5. Scott Q. Harper, Pedro Gonzalez-Alegre
      Pages 95-112
    6. Tsuyoshi Ohkumo, Chikahide Masutani, Toshihiko Eki, Fumio Hanaoka
      Pages 129-137
    7. Qingquan Tang, Baojian Li, Martin Woodle, Patrick Y. Lu
      Pages 139-158
    8. Wenyi Gu, Lisa Putral, Nigel McMillan
      Pages 159-172
    9. Toshiaki Watanabe, Hiroshi Imai, Naojiro Minami
      Pages 173-185
    10. Sumona Karjee, Mohammad Nurul Islam, Sunil K. Mukherjee
      Pages 187-203
    11. Michael E. Scharf, Xuguo Zhou, Margaret A. Schwinghammer
      Pages 205-229
    12. Thomas Baeriswyl, Olivier Mauti, Esther T. Stoeckli
      Pages 231-244
    13. Pumin Zhang
      Pages 259-266
  4. Back Matter
    Pages 267-270

About this book


RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology.  In RNAi: Design and Applications, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research.  Divided into two parts, this comprehensive volume covers fundamentals including designs of RNAi, biochemical assay protocols for the major components of RNAi, and study of potential off-target effects, followed by an extensive section covering various applications of RNAi in diverse model organisms and systems, from antiviral and anticancer applications to altering flower color in plants. 

Cutting edge and clearly written, RNAi: Design and Applications enables a researcher with standard molecular biological training to perform major RNAi-related experiments and contribute to this revolutionary, growing field.


Evolution Microarray RNA interference currentsara genes miRNA siRNA

Editors and affiliations

  • Sailen Barik
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyUniversity of South AlabamaMobile

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-874-4
  • Online ISBN 978-1-59745-191-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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