Hematopoietic Stem Cell Protocols

  • Kevin D. Bunting

Part of the Methods in Molecular Biology™ book series (MIMB, volume 430)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Overview

    1. Front Matter
      Pages 1-1
    2. William Tse, Kevin D. Bunting
      Pages 3-18
  3. Stem Cell Enrichment and Analysis

    1. Front Matter
      Pages 19-19
    2. Simon N. Robinson, Ronald P. van Os
      Pages 31-53
    3. Seiji Fukuda, Louis M. Pelus
      Pages 55-75
  4. In Vitro Assays and Differentiation

    1. Front Matter
      Pages 101-101
    2. Xinghui Tian, Dan S. Kaufman
      Pages 119-133
    3. Génève Awong, Ross N. La Motte-Mohs, Juan Carlos Zúñiga-Pflücker
      Pages 135-142
    4. Ronald P. van Os, Bertien Dethmers-Ausema, Gerald de Haan
      Pages 143-157
  5. Transplantation Assays for Mouse and Human HSC

    1. Front Matter
      Pages 159-159
    2. Jonathan B. Johnnidis, Fernando D. Camargo
      Pages 183-193
    3. Sigrid Eckardt, K. John McLaughlin
      Pages 195-211
    4. Ping Zhou, Sarah Hohm, Ben Capoccia, Louisa Wirthlin, David Hess, Dan Link et al.
      Pages 213-225
  6. Genetic Modification of HSCs and Imaging Engraftment

    1. Front Matter
      Pages 227-227
    2. Peter Haviernik, Yi Zhang, Kevin D. Bunting
      Pages 229-241
    3. Zhaohui Ye, Xiaobing Yu, Linzhao Cheng
      Pages 243-253
    4. Olga S. Kustikova, Christopher Baum, Boris Fehse
      Pages 255-267
    5. Yuan Lin, Joe Molter, Zhenghong Lee, Stanton L. Gerson
      Pages 295-306
  7. Back Matter
    Pages 307-312

About this book


With new technologies emerging and old technologies being reimagined, the field of hematopoietic stem cell (HSC) research continues to grow rapidly in pursuit of improved therapies for many blood diseases. Updating and building upon the thorough first edition's foundation, Hematopoietic Stem Cell Protocols, Second Edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. An introductory chapter also hints at potential techniques that may develop in the future. Following the format of the successful Methods in Molecular Biology™ series, each protocol offers step-by-step laboratory instructions, lists of the necessary equipment, and tips on troubleshooting and avoiding known pitfalls.

Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Second Edition is a much needed technical resource in the critically important field of stem cell investigation.


BAC Flow cytometry HSC Lentiviral transduction Migration Retroviral transduction Stem cell investigation genes molecular biology tissue

Editors and affiliations

  • Kevin D. Bunting
    • 1
  1. 1.Department of Medicine, Division of Hematology & OncologyCenter for Stem Cell and Regenerative Medicine, Case Western Reserve UniversityClevelandUSA

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-868-3
  • Online ISBN 978-1-59745-182-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Chemical Manufacturing
Health & Hospitals
Consumer Packaged Goods