HIV Protocols

  • Vinayaka R. Prasad
  • Ganjam V. Kalpana

Part of the Methods In Molecular Biology™ book series (MIMB, volume 485)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Preparation of Virus Particles and their Analysis

    1. Front Matter
      Pages 1-1
    2. Kimdar Sherefa Kemal, Milan Reinis, Barbara Weiser, Harold Burger
      Pages 3-14
    3. Yong Gao, Immaculate Nankya, Awet Abraha, Ryan M. Troyer, Kenneth N. Nelson, Andrea Rubio et al.
      Pages 27-35
  3. Methods To Study Hiv-1 Replication

    1. Front Matter
      Pages 36-37
    2. Early Events

      1. Christopher Aiken
        Pages 41-53
      2. Jean L. Mbisa, Krista A. Delviks-Frankenberry, James A. Thomas, Robert J. Gorelick, Vinay K. Pathak
        Pages 55-72
      3. Dibyakanti Mandal, Vinayaka R. Prasad
        Pages 73-85
      4. Michael D. Moore, Mario P. S. Chin, Wei-Shau Hu
        Pages 87-105
      5. Nathalie J. Arhel, Pierre Charneau
        Pages 151-159
    3. Late Events

      1. Abdul A. Waheed, Akira Ono, Eric O. Freed
        Pages 163-184
      2. Jaisri R. Lingappa, Beth K. Thielen
        Pages 185-195
  4. Specialized Approaches To Study Hiv-1 Biology and Pathogenesis

    1. Front Matter
      Pages 233-233
    2. Susana T. Valente, Stephen P. Goff
      Pages 235-255
    3. Manuel Llano, Natassia Gaznick, Eric M. Poeschla
      Pages 257-270
    4. Vasudev R. Rao, Eliseo A. Eugenin, Joan W. Berman, Vinayaka R. Prasad
      Pages 295-309
    5. Aviva Joseph, Kaori Sango, Harris Goldstein
      Pages 311-327
  5. Immunological Studies Of Hiv

    1. Front Matter
      Pages 329-329
    2. Mucosal Immunology

      1. Zina Moldoveanu, Jiri Mestecky
        Pages 333-345
      2. Barbara L. Shacklett, J. William Critchfield, Donna Lemongello
        Pages 347-356
    3. Measuring T Cell Responses via Flow Cytometry

      1. Barbara L. Shacklett, J. William Critchfield, Donna Lemongello
        Pages 359-374
    4. Antiviral Responses

  6. Drug Resistant Viruses And Viral Evolution

    1. Front Matter
      Pages 425-425
    2. Maureen Oliveira, Bluma G. Brenner, Mark A. Wainberg
      Pages 427-433
    3. Ben Berkhout, Atze T. Das
      Pages 436-451
  7. Back Matter
    Pages 453-457

About this book


Despite major advances in the treatment of HIV, there are still many areas requiring additional study. These areas include ways to create efficacious, potent antiretrovirals for suppressing viral loads completely without toxic side effects, the definition of unknown drug targets while fine-tuning known targets, and the ability to better understand the interplay between viral and host factors. In HIV Protocols, Second Edition, expert researchers provide clear, state-of-the-art methods for the study of HIV. Directed toward three specific goals, this text aims to document up-to-date protocols for select aspects of HIV biology, to bring together both virological and immunological approaches in a single, convenient volume, and to present a comprehensive account of a range of techniques not available in any existing HIV protocol book. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections containing priceless tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, HIV Protocols, Second Edition is an ideal guide to the wide array of techniques used in fundamental or applied research into the biology and pathogenesis of HIV-1.


AIDS vaccine Antiretrovirals HIV HIV biology HIV-1 replication Immunological studies Pathogenesis Viral evolution Virus Virus particle analysis infectious

Editors and affiliations

  • Vinayaka R. Prasad
    • 1
  • Ganjam V. Kalpana
    • 1
  1. 1.Albert Einstein College of MedicineBronxUSA

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-859-1
  • Online ISBN 978-1-59745-170-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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