© 2007

Flow Cytometry in Hematopathology

A Visual Approach to Data Analysis and Interpretation


  • Step-by-step methods for flow cytometry data analysis and interpretation

  • Emphasis on visual pattern analysis

  • Coverage of acute leukemias, lymphoproliferative disorders and myelodysplastic syndromes

  • Extensive FCM graphics, including minimal residual disease detection in ALL, TCR-Vb repertoire in mature T-cell disorders and DNA content analysis in ALL and malignant lymphomas


About this book


Although instrumentation and laboratory techniques for flow cytometry (FCM) immunophenotyping of hematopoietic malignancies are well documented, there is relatively little information on how best to perform data analysis, a critical step in FCM testing. In Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation, three physicians highly experienced in laboratory hematopathology and FCM offer a unique systematic approach to FCM data analysis and interpretation based on the visual inspection of dual parameter FCM graphics. This step-by-step approach to optimal FCM data analysis is demonstrated by means of numerous FCM graphics derived from actual well-documented clinical cases. In this second edition of this well received book, the authors have revised and expanded the text and added more than 100 figures to reflect the recent advances in the field. The focus of the additional material is on the TCR-Vb eight-tube kit which has greatly facilitated the evaluation mature T-cell disorders, and on the DNA dye DRAQ5 for improved grading of malignant lymphoma. The authors also include notes on "tricks of the trade" and pitfalls to avoid. The discussion, covering leukemias, lymphomas, and other conditions, moves from simple to complex specimens, with an emphasis on visual pattern analysis.
Richly illustrated and highly instructive, Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation offers clinical pathologists, hematopathologists, and specialists in laboratory medicine a much-needed guide with a practical and logical approach for sharpening their FCM data analysis skills on a wide spectrum of hematologic disorders.


Laboratory blood bone marrow cell lymphocytes lymphoma plasma tissue

Authors and affiliations

  1. 1.University of Florida College of MedicineGainesville

Bibliographic information

  • Book Title Flow Cytometry in Hematopathology
  • Book Subtitle A Visual Approach to Data Analysis and Interpretation
  • Authors Doyen T. Nguyen
    Lawrence W. Diamond
    Raul C. Braylan
  • DOI
  • Copyright Information Humana Press Inc., a part of Springer Science+Business Media, LLC 2007
  • Publisher Name Humana Press
  • eBook Packages Medicine Medicine (R0)
  • Hardcover ISBN 978-1-58829-855-3
  • Softcover ISBN 978-1-62703-911-6
  • eBook ISBN 978-1-59745-162-8
  • Edition Number 2
  • Number of Pages XIX, 344
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Pathology
    Laboratory Medicine
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals


"The first edition of this book was wonderful, and this updated edition continues the tradition of excellence.  You need this book for ready access if you do hematopathology." -Doody's Book Review, Weighted Numerical Score:96 - 4 Stars

From the reviews of the second edition:

"The book is nicely illustrated and contains numerous colour figures illustrating the broad variety of flow cytometry applications in various haematological conditions. … The book is very well suited for clinical pathologists, assistants in training in the field of laboratory medicine and clinical pathology, medical technicians and researchers working in clinical haematology laboratories, as well as clinical haematologists who want to get familiar with the recent evolutions in … diagnostic laboratory medicine." (Joris Delanghe, Acta Clinica Belgica, Vol. 63 (2), 2008)