Flow Cytometry in Hematopathology

A Visual Approach to Data Analysis and Interpretation

  • Doyen Nguyen
  • Lawrence W. Diamond
  • Raul C. Braylan

About this book


Although instrumentation and laboratory techniques for flow cytometry (FCM) immunophenotyping of hematopoietic malignancies are well documented, there is relatively little information on how best to perform data analysis, a critical step in FCM testing. In Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation, three physicians highly experienced in laboratory hematopathology and FCM offer a unique systematic approach to FCM data analysis and interpretation based on the visual inspection of dual parameter FCM graphics. This step-by-step approach to optimal FCM data analysis is demonstrated by means of numerous FCM graphics derived from actual well-documented clinical cases. In this second edition of this well received book, the authors have revised and expanded the text and added more than 100 figures to reflect the recent advances in the field. The focus of the additional material is on the TCR-Vb eight-tube kit which has greatly facilitated the evaluation mature T-cell disorders, and on the DNA dye DRAQ5 for improved grading of malignant lymphoma. The authors also include notes on "tricks of the trade" and pitfalls to avoid. The discussion, covering leukemias, lymphomas, and other conditions, moves from simple to complex specimens, with an emphasis on visual pattern analysis.
Richly illustrated and highly instructive, Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation offers clinical pathologists, hematopathologists, and specialists in laboratory medicine a much-needed guide with a practical and logical approach for sharpening their FCM data analysis skills on a wide spectrum of hematologic disorders.


Laboratory blood bone marrow cell lymphocytes lymphoma plasma tissue

Authors and affiliations

  • Doyen Nguyen
    • 1
  • Lawrence W. Diamond
    • 1
  • Raul C. Braylan
    • 1
  1. 1.University of Florida College of MedicineGainesville

Bibliographic information

  • DOI
  • Copyright Information Humana Press Inc., a part of Springer Science+Business Media, LLC 2007
  • Publisher Name Humana Press
  • eBook Packages Medicine
  • Print ISBN 978-1-58829-855-3
  • Online ISBN 978-1-59745-162-8
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals