Nuclear Transfer Protocols

Cell Reprogramming and Transgenesis

  • Paul J. Verma
  • Alan O. Trounson

Part of the Methods in Molecular Biology™ book series (MIMB, volume 348)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Nuclear Transfer

    1. Front Matter
      Pages 197-197
    2. Culture and Maintenance of Nuclear Transfer Embryos

      1. Marc-André Sirard, Karine Coenen
        Pages 35-42
      2. Zoltán Macháty
        Pages 43-58
      3. Björn Heindryckx, Josiane Van der Elst, Marc Dhont
        Pages 59-77
      4. Tim King, Paul A. De Sousa
        Pages 79-90
      5. Jacqui T. Forsyth, David N. Wells
        Pages 91-108
    3. Nuclear Transfer Methods

      1. Young Gie Chung, Shaorong Gao, Keith E. Latham
        Pages 111-123
      2. Kazuchika Miyoshi, Sezen Arat, Steven L. Stice
        Pages 125-134
      3. Irina A. Polejaeva, Shawn Walker, Keith Campbell
        Pages 135-149
      4. Shoukhrat M. Mitalipov, Don P. Wolf
        Pages 151-168
      5. James “Buck” Williams, Taeyoung Shin, Ling Liu, Gabriela Flores-Foxworth, Juan Romano, Alice Blue-McClendon et al.
        Pages 169-181
      6. Gábor Vajta, Ian M. Lewis, R. Tayfur Tecirlioglu
        Pages 183-195
    4. Trangenesis

      1. A. John Clark, Sarah Burl, Chris Denning
        Pages 199-212
      2. Athoula Lazaris, Rebecca Keyston, Costas N. Karatzas, Carol L. Keefer
        Pages 213-226
  3. Reprogramming Nuclei

    1. Front Matter
      Pages 227-227
    2. Takashi Tada
      Pages 229-236
    3. Takashi Kohda, Fumitoshi Ishino, Atsuo Ogura
      Pages 237-246
    4. Koichi Gonda, Nobuaki Kikyo
      Pages 247-258
    5. Anne-Mari Håkelien, Thomas Küntziger, Kristine G. Gaustad, Anne Marstad, Philippe Collas
      Pages 259-267
    6. Danièle Pralong, Paul J. Verma
      Pages 269-283
    7. Christine Wrenzycki, Doris Herrmann, Claudia Gebert, Joseph W. Carnwath, Heiner Niemann
      Pages 285-303
  4. Concluding Remarks

    1. Front Matter
      Pages 305-305
    2. Ian Wilmut
      Pages 307-318
    3. Alan O. Trounson
      Pages 319-331
  5. Back Matter
    Pages 333-337

About this book


Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones.


Activation gene expression gene transfer genes reproducible techniques vertebrates

Editors and affiliations

  • Paul J. Verma
    • 1
  • Alan O. Trounson
    • 2
  1. 1.Centre for Reproduction and Development Monash Institute of Medical ResearchMonash UniversityMelbourneAustralia
  2. 2.Monash Immunology and Stem Cell LaboratoriesMonash UniversityMelbourneAustralia

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-280-3
  • Online ISBN 978-1-59745-154-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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