Neural Stem Cells

Methods and Protocols

  • Leslie P. Weiner

Part of the Methods in Molecular Biology™ book series (MIMB, volume 438)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Leslie P. Weiner
      Pages 3-8
  3. Isolation and Culture of NSCs

    1. Front Matter
      Pages 17-17
    2. Mirella Dottori, Martin F. Pera
      Pages 19-30
    3. Andreas Androutsellis-Theotokis, Sachiko Murase, Justin D. Boyd, Deric M. Park, Daniel J. Hoeppner, Rea Ravin et al.
      Pages 31-38
    4. Yuanyuan Wu, Ying Liu, Jonathan D. Chesnut, Mahendra S. Rao
      Pages 39-53
    5. Rossella Galli, Angela Gritti, Angelo L. Vescovi
      Pages 67-84
    6. Miranda Richter, Kathryn Westendorf, A. Jane Roskams
      Pages 95-102
    7. Seung U. Kim, Atsushi Nagai, Eiji Nakagawa, Hyun B. Choi, Jung H. Bang, Hong J. Lee et al.
      Pages 103-121
    8. Gregory P. Marshall, Heather H. Ross, Oleg Suslov, Tong Zheng, Dennis A. Steindler, Eric D. Laywell
      Pages 135-150
    9. Shigeo Saito, Ken Sawai, Yoshinobu Murayama, Keiichi Fukuda, Kazunari Yokoyama
      Pages 151-169
  4. In Vitro Studies: A. Cellular Techniques

    1. Front Matter
      Pages 171-171
    2. Angela Gritti, Rossella Galli, Angelo L. Vescovi
      Pages 173-184
    3. Mark P. Mattson, Peisu Zhang, Aiwu Cheng
      Pages 185-196
    4. Suzanne E. Peterson, Jurjen W. Westra, Christine M. Paczkowski, Jerold Chun
      Pages 197-204
    5. Pascale Durbec, Isabelle Franceschini, Francoise Lazarini*, Monique Dubois-Dalcq
      Pages 213-225
    6. Brett T. Lund, Eve E. Kelland
      Pages 227-241
    7. Eric Wexler
      Pages 243-268
  5. In Vitro Studies: B. Molecular Techniques

  6. Studies for Transplantation

    1. Front Matter
      Pages 333-333
    2. Sanjay S. Magavi, Jeffrey D. Macklis
      Pages 345-352
    3. Lisa A. Catapano, Sanjay S. Magavi, Jeffrey D. Macklis
      Pages 353-359
    4. Scott R. Whittemore, Yi Ping Zhang, Christopher B. Shields, David S.K. Magnuson
      Pages 375-382
    5. Alison E. Willing, Svitlana Garbuzova-Davis, Paul R. Sanberg, Samuel Saporta
      Pages 383-401
  7. Back Matter
    Pages 403-406

About this book


Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger.  Neural Stem Cells, 2nd Edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions.  The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models.  In addition to the topics covered in the first edition, this new volume provides recently developed alternative methods for both human and rodent NSCs, along with chapters on karyotyping, migration, apoptosis, and NSC markers. Following the Methods in Molecular Biology™ series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and current, Neural Stem Cells, 2nd Edition is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.


Gene expression studies NSC Neurodegenerative diseases Neurogenetic diseases PCR Telomere Transplantation cell lines cryopreservation gene expression genes molecular biology neurons phenotype single-cell polymerase chain reaction

Editors and affiliations

  • Leslie P. Weiner
    • 1
  1. 1.Keck School of MedicineUniversity of Southern CaliforniaLos AngelesUSA

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-846-1
  • Online ISBN 978-1-59745-133-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site