Clinical Proteomics

Methods and Protocols

  • Antonia Vlahou

Part of the Methods in Molecular Biology™ book series (MIMB, volume 428)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Specimen Collection for Clinical Proteomics

    1. Front Matter
      Pages 45-45
    2. Young-Ki Paik, Hoguen Kim, Eun-Young Lee, Min-Seok Kwon, Sang Yun Cho
      Pages 1-31
    3. Harald Tammen
      Pages 35-42
    4. Jose I. Diaz, Lisa H. Cazares, O. John Semmes
      Pages 43-53
  3. Clinical Proteomics by 2DE and Direct MALDI/SELDI MS Profiling

    1. Front Matter
      Pages 65-65
    2. Sang Yun Cho, Eun-Young Lee, Hye-Young Kim, Min-Jung Kang, Hyoung-Joo Lee, Hoguen Kim et al.
      Pages 57-75
    3. David B Friedman, Kathryn S Lilley
      Pages 93-124
    4. Lisa H Cazares, Jose I Diaz, Rick R Drake, O John Semmes
      Pages 125-140
    5. Dana Mustafa, Johan M. Kros, Theo Luider
      Pages 159-178
  4. Clinical Proteomics by LC-MS Approaches

    1. Front Matter
      Pages 185-185
    2. Chen Li, Yi-Hong, Ye-Xiong Tan, Jian-Hua Ai, Hu Zhou, Su-Jun Li et al.
      Pages 193-208
    3. Richard E. Higgs, Michael D. Knierman, Valentina Gelfanova, Jon P. Butler, John E. Hale
      Pages 209-230
    4. Zhen Xiao, Thomas P Conrads, George R Beck Jr, Timothy D Veenstra
      Pages 231-244
  5. Clinical Proteomics and Antibody Arrays

    1. Front Matter
      Pages 250-250
    2. Hsin-Yun Hsu, Silke Wittemann, Thomas O. Joos
      Pages 247-261
  6. Statistics and Bioinformatics in Clinical Proteomics Data Analysis

    1. Front Matter
      Pages 291-291
    2. Emilio Marengo, Elisa Robotti, Marco Bobba
      Pages 291-325
    3. Sebastien Christian Carpentier, Bart Panis, Rony Swennen, Jeroen Lammetryn
      Pages 327-347
    4. Costas D. Paliakasis, Ioannis Michalopoulos, Sophia Kossida
      Pages 349-367
    5. Matthew Fitzgibbon, Wendy Law, Damon May, Andrea Detter, Martin McIntosh
      Pages 369-381
  7. Back Matter
    Pages 397-404

About this book


The field of clinical proteomics has rapidly evolved during the past few years and is continuously growing as new methodologies and technologies emerge. In Clinical Proteomics, a select group of leading researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease biomarkers in tissues, microdissected cells and body fluids. Experimental approaches involving the application of two-dimensional electrophoresis, multi-dimensional liquid chromatography, SELDI/MALDI mass spectrometry and protein arrays, as well as the bioinformatic and statistical tools pertinent to the analysis of proteomics data, are thoroughly detailed in these readily reproducible methods and protocols, explained through the step-by-step instructional format of the highly successful Methods in Molecular Biology™ series.

With its multi-disciplinary approach, Clinical Proteomics provides integrated, cutting-edge techniques sure to be invaluable to a wide range of researchers including clinicians, molecular biologists, chemists, bioinformaticians and computational biologists.


2D electrophoresis Clinical proteomics Computational biology Polyacrylamid-Gelelektrophorese Proteomics biochemistry bioinformatics biomarkers cells molecular biology protein profiling tissue

Editors and affiliations

  • Antonia Vlahou
    • 1
  1. 1.Biomedical Research FoundationAcademy of AthensAthensGreece

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-837-9
  • Online ISBN 978-1-59745-117-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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