Methods and Protocols

  • Jennifer J. Westendorf

Part of the Methods In Molecular Biology™ book series (MIMB, volume 455)

Table of contents

  1. Front Matter
    Pages i-xix
  2. In Vitro Models

    1. Thomas A. Owen, Lydia C. Pan
      Pages 3-18
    2. Elizabeth W. Bradley, Merry Jo Oursler
      Pages 19-35
    3. Khalid S. Mohammad, John M. Chirgwin, Theresa A. Guise
      Pages 37-50
    4. Teresita Bellido, Lilian I. Plotkin
      Pages 51-75
  3. In Vivo Rodent Models

    1. Li Xin, Qin Ling, C. Partridge Nicola
      Pages 79-87
    2. Glenda J. Pettway, Laurie K. McCauley
      Pages 101-110
    3. Yogendra P. Kharode, Michael C. Sharp, Peter V.N. Bodine
      Pages 111-124
    4. Stéphane Blouin, Hélène Libouban, Marie Françoise Moreau, Daniel Chappard
      Pages 125-134
  4. Methods of Gene Delivery

    1. Renny T. Franceschi, Chunxi Ge
      Pages 137-147
    2. Marc N. Wein, Dallas C. Jones, Laurie H. Glimcher
      Pages 149-155
    3. Valerie Deregowski, Ernesto Canalis
      Pages 157-162
  5. Molecular and Genetic Techniques

    1. Margaretha van der Deen, Mohammad Q. Hassan, Jitesh Pratap, Nadiya M. Teplyuk, Daniel W. Young, Amjad Javed et al.
      Pages 165-176
    2. Artem Barski, Steven Pregizer, Baruch Frenkel
      Pages 177-190
    3. Dong-Hai Xiong, Jian-Feng Liu, Yan-Fang Guo, Yan Guo, Tie-Lin Yang, Hui Jiang et al.
      Pages 203-235
  6. Imaging Techniques

    1. Shirwin M. Pockwinse, Sayyed K. Zaidi, Ricardo F. Medina, Rachit Bakshi, Krishna P. Kota, Syed A. Ali et al.
      Pages 239-259
    2. Yoram Zilberman, Yossi Gafni, Gadi Pelled, Zulma Gazit, Dan Gazit
      Pages 261-272
    3. Samuel Gourion-Arsiquaud, Paul A. West, Adele L. Boskey
      Pages 293-303
  7. Mechanical Testing

    1. Leanne Kaye Saxon, Lance Edward Lanyon
      Pages 307-322
    2. Engin Ozcivici, Suzanne Ferreri, Yi-Xian Qin, Stefan Judex
      Pages 323-334
    3. Ryan C. Riddle, Amanda F. Taylor, Henry J. Donahue
      Pages 335-345
  8. Back Matter
    Pages 347-354

About this book


In recent years, the study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field.  In Osteoporosis: Methods and Protocols, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology.  The topics covered in this volume include in vitro models, in vivo models utilized for drug testing, tissue engineering and osteoporosis studies in either gender, state-of-the-art molecular techniques to assess single genes or for global genomic analysis, powerful imaging techniques, and many more.  As a volume in the highly successful Methods in Molecular Biology™ series, each chapter provides a brief introduction, a list of necessary materials, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Osteoporosis: Methods and Protocols is the perfect guide for both new and experienced experimentalists attempting to study the devastating silent disease known as osteoporosis.


Bone biology Bone cells Bone tissue In vitro and in vivo models Osteoblasts and Osteoclasts Termination computed tomography (CT) genes imaging techniques molecular biology tissue transcription

Editors and affiliations

  • Jennifer J. Westendorf
    • 1
  1. 1.Department of Orthopedic SurgeryMayo ClinicRochesterUSA

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-828-7
  • Online ISBN 978-1-59745-104-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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