Methods and Protocols

  • Niclas G. Karlsson
  • Nicolle H. Packer

Part of the Methods in Molecular Biology™ book series (MIMB, volume 534)

Table of contents

  1. Front Matter
    Pages ii-xiv
  2. Glycan Analysis

    1. Front Matter
      Pages 1-2
    2. Willy Morelle, Valegh Faid, Frédéric Chirat, Jean-Claude Michalski
      Pages 3-21
    3. Bensheng Li, Hyun Joo An, Jerry L. Hedrick, Carlito B. Lebrilla
      Pages 23-35
    4. Parastoo Azadi, Christian Heiss
      Pages 37-51
    5. Yehia Mechref, Pilsoo Kang, Milos V. Novotny
      Pages 53-64
    6. Kazuaki Kakehi, Mitsuhiro Kinoshita
      Pages 93-105
    7. Hasse Karlsson, Jessica M. Holmén Larsson, Kristina A. Thomsson, Iris Härd, Malin Bäckström, Gunnar C. Hansson
      Pages 117-131
    8. Bensheng Li, Hyun Joo An, Jerry L. Hedrick, Carlito B. Lebrilla
      Pages 133-145
    9. Vikas Prabhakar, Ishan Capila, Ram Sasisekharan
      Pages 147-156
    10. Ruby P. Estrella, John M. Whitelock, Rebecca H. Roubin, Nicolle H. Packer, Niclas G. Karlsson
      Pages 171-192
  3. Glycopeptides

    1. Front Matter
      Pages 1-1
    2. Shigeyasu Ito, Ko Hayama, Jun Hirabayashi
      Pages 194-203
    3. Nicole Wilson, Raina Simpson, Catherine Cooper-Liddell
      Pages 205-212
    4. Bing Gong, Michael Cukan, Richard Fisher, Huijuan Li, Terrance A. Stadheim, Tillman Gerngross
      Pages 213-223
  4. O-GlcNAcomics

  5. Glycobioinformatic Tools

    1. Front Matter
      Pages 1-1
    2. Hiromi Ito, Akihiko Kameyama, Takashi Sato, Hisashi Narimatsu
      Pages 282-291
    3. Thomas Lütteke, Claus W. von der Lieth
      Pages 293-310
  6. Carbohydrate-Protein Interactions

    1. Front Matter
      Pages 1-1
    2. Andrew K. Powell, Zheng-liang Zhi, Jeremy E. Turnbull
      Pages 312-329
    3. Vikas Prabhakar, Ishan Capila, Ram Sasisekharan
      Pages 331-340
    4. Jamie Heimburg-Molinaro, Kate Rittenhouse-Olson
      Pages 341-357
  7. Back Matter
    Pages 387-389

About this book


Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Glycomics: Methods and Protocols serves as a valuable guide for experimenters facing the challenges of glycan analysis in hope of providing further insights into the biology of cell-cell communication and interaction.


Microarray Oligosaccharid Polyacrylamid-Gelelektrophorese Termination molecular biology proteins proteoglycans

Editors and affiliations

  • Niclas G. Karlsson
  • Nicolle H. Packer

There are no affiliations available

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-774-7
  • Online ISBN 978-1-59745-022-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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