© 2003

Handbook of Proteomic Methods

  • P. Michael Conn

Table of contents

  1. Front Matter
    Pages i-xiv
  2. General Techniques

    1. Front Matter
      Pages 1-1
    2. Paul R. Graves, Timothy A. J. Haystead
      Pages 3-16
    3. Nathalie Norais, Renzo Nogarotto, Emilia Tiziana Iacobini, Ignazio Garaguso, Renata Grifantini, Giuliano Galli et al.
      Pages 17-28
    4. Shanhua Lin, Ning Tang, Scot R. Weinberger
      Pages 59-72
    5. Karen J. Martin, Wayne F. Patton
      Pages 93-105
    6. Birte Schulenberg, Wayne F. Patton
      Pages 107-115
    7. Alexandra Huhalov, Daniel I. R. Spencer, Kerry A. Chester
      Pages 117-127
    8. Ian I. Stewart, Ty Thomson, Daniel Figeys, Henry S. Duewel
      Pages 145-179
    9. Bart Devreese, Frank Vanrobaeys, Elke Lecocq, Joél Smet, Rudy Van Coster, Jozef Van Beeumen
      Pages 181-191
    10. Joel R. Bock, David A. Gough
      Pages 193-222
  3. Post-Translational Modifications, Variants, and Isoforms

    1. Front Matter
      Pages 223-223
    2. Catherine A. Cooper, Elisabeth Gasteiger, Nicolle H. Packer
      Pages 225-231
    3. Catherine A. Cooper, Hiren J. Joshi, Mathew J. Harrison, Marc R. Wilkins, Nicolle H. Packer
      Pages 233-239
    4. Li-Rong Yu, Van M. Hoang, Timothy D. Veenstra
      Pages 241-257
    5. Frédéric Nikitin, Frédérique Lisacek
      Pages 259-268

About this book


The developing new field of proteomics holds significant promise for a deeper understanding of complicated biological systems, as well as for novel approaches to the cure of disease. In the Handbook of Proteomic Methods, P. Michael Conn has assembled a panel of well-recognized experts to describe a wide range of powerful techniques for identifying and analyzing the diversity of proteins expressed in cells. These readily reproducible proteomic methods range from general to specific techniques, and include methods for data analysis, post-translational modification, and its variants and isoforms. Additional methods demonstrate the application of proteomics to the discovery of serological tumor markers, to identifying the determinants of sensitivity to antitumor drugs, and to specialized fields, such as endocrinology, plant biology, nephrology, and urology. Each fully tested protocol is described in step-by-step detail, complete with tricks of the trade and hints on avoiding pitfalls. The detail of description exceeds that usually found in primary publications.
Wide-ranging and practical, the Handbook of Proteomic Methods offers scientists entering, or already active in, the field a fully referenced compendium of easy-to-use proteomic methods-each reflecting the power and promise of proteomics in illuminating complex biological systems and in promoting the development of novel drugs for the amelioration of disease.


Amino acid Proteomics proteins transcription translation

Editors and affiliations

  • P. Michael Conn
    • 1
  1. 1.Oregon National Primate Research CenterOregon Health and Science UniversityBeavertonUSA

Bibliographic information

  • Book Title Handbook of Proteomic Methods
  • Editors P. Michael Conn
  • DOI
  • Copyright Information Springer Science+Business Media New York 2003
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Book Archive
  • Hardcover ISBN 978-1-58829-340-4
  • Softcover ISBN 978-1-61737-504-0
  • eBook ISBN 978-1-59259-414-6
  • Edition Number 1
  • Number of Pages XIV, 510
  • Number of Illustrations 129 b/w illustrations, 7 illustrations in colour
  • Topics Biochemistry, general
  • Buy this book on publisher's site
Industry Sectors
Chemical Manufacturing
Health & Hospitals
Consumer Packaged Goods


"...covers recent advances and also acts as a manual of well-practiced techniques. This volume should appeal particularly to the uninitiated but contains several articles of interest to more experienced practitioners...As well as being an excellent guide, this volume in its entirety makes a strong case for the advantages in the application of these post-genomic technologies" - Rapid Communications in Mass Spectrometry

"Handbook of Proteomic Methods includes a wide range of techniques for identifying and analyzing proteins expressed in cells. As many other Humana Press method books, this is another must-have for large academic libraries supporting research in complex biological systems and drug development." -E-STREAMS

"This is a very well produced volume with excellent diagrams and reproduction of difficult images such as 2-D gels...deserves a place on the proteomic researchers' bookshelf." - Chromatographia