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Macrophage Migration Inhibitory Factor

Methods and Protocols

  • James Harris
  • Eric F. Morand
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2080)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Adrian Hoffmann, Leon Christian Zwißler, Omar El Bounkari, Jürgen Bernhagen
    Pages 1-18
  3. Fabien B. Vincent, Tali Lang
    Pages 47-56
  4. Kirstin D. Elgass, Sarah J. Creed, Ina Rudloff
    Pages 93-114
  5. Jawad H. Abidi, James Harris, Nadia S. Deen
    Pages 115-122
  6. Nadia S. Deen, Jacinta P. Lee, James Harris
    Pages 147-158
  7. Venkata Sita Rama Raju Allam, Maria B. Sukkar
    Pages 203-212
  8. Izabela Galvão, Allysson Cramer, Flavio Almeida Amaral
    Pages 213-222
  9. Dzmitry Sinitski, Katrin Gruner, Jürgen Bernhagen, Ralph Panstruga
    Pages 249-261
  10. Back Matter
    Pages 263-265

About this book

Introduction

This volume looks at--and discusses the techniques of--a range of areas in biology in which macrophage migration inhibitory factor (MIF) is studied. Topics covered in this book include methods to quantify MIF in cell supernatants and human samples, using ELISA and western blot; detection and analysis of intracellular MIF using flow cytometry; genotyping of human MIF polymorphisms, detection of MIF expression in a marine invertebrate using rapid amplification of cDNA RACE; studying the MIF receptor CD74 in tissues using immunohistochemistry; and studying MIF in models of disease such as asthma, Plasmodium infection, and gout. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Comprehensive and practical, Macrophage Migration Inhibitory Factor: Methods and Protocols is a valuable resource that will help researchers gain a new understanding of MIF biology in health and disease. 

Keywords

Cell death CRISPR siRNA autophagy Mass spectrometry genotyping

Editors and affiliations

  • James Harris
    • 1
  • Eric F. Morand
    • 2
  1. 1.Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical CentreMonash UniversityClaytonAustralia
  2. 2.Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical CentreMonash UniversityClaytonAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-9936-1
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9935-4
  • Online ISBN 978-1-4939-9936-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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