Antibody-Drug Conjugates

Methods and Protocols

  • L. Nathan Tumey

Part of the Methods in Molecular Biology book series (MIMB, volume 2078)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Michael J. McPherson, Adrian D. Hobson
    Pages 23-36
  3. Durgesh V. Nadkarni
    Pages 37-49
  4. Pragya Adhikari, Neelie Zacharias, Rachana Ohri, Jack Sadowsky
    Pages 51-69
  5. Yasuaki Anami, Kyoji Tsuchikama
    Pages 71-82
  6. Tak Ian Chio, Susan L. Bane
    Pages 83-97
  7. Matthew Bird, Joao Nunes, Mark Frigerio
    Pages 113-129
  8. Hanna Toftevall, Helén Nyhlén, Fredrik Olsson, Jonathan Sjögren
    Pages 131-145
  9. Soraya Chapel, Florent Rouvière, Morgan Sarrut, Sabine Heinisch
    Pages 163-185
  10. Elsa Wagner-Rousset, Olivier Colas, Yannis-Nicolas François, Sabine Heinisch, Davy Guillarme, Sarah Cianférani et al.
    Pages 187-195
  11. Oscar Hernandez-Alba, Anthony Ehkirch, Alain Beck, Sarah Cianférani
    Pages 197-211
  12. Jintang He, Surinder Kaur, Keyang Xu
    Pages 213-219
  13. Linjie Han, Yanqun Zhao, Qunying Zhang
    Pages 221-233
  14. Hua Sang, Ning Wan, Gaoyuan Lu, Yang Tian, Guangji Wang, Hui Ye
    Pages 235-250
  15. Wenjing Ning, Yanqun Zhao
    Pages 251-262
  16. Josiane Saadé, Rabah Gahoual, Alain Beck, Emmanuelle Leize-Wagner, Yannis-Nicolas François
    Pages 263-272
  17. Calvin L. Becker, Robert J. Duffy, Jorge Gandarilla, Steven M. Richter
    Pages 273-290
  18. Jeffrey Casavant, Anokha S. Ratnayake, Sujiet Puthenveetil, L. Nathan Tumey
    Pages 291-299
  19. Vamsi Krishna Mudhivarthi, Jianxin Guo
    Pages 313-327
  20. Shengjia Wu, Dhaval K. Shah
    Pages 329-340
  21. Andrew J. Bessire, Chakrapani Subramanyam
    Pages 341-351
  22. Hsuan-Ping Chang, Dhaval K. Shah
    Pages 361-369
  23. Back Matter
    Pages 371-373

About this book


This volume looks at key methodologies that are commonly used across antibody drug conjugates (ADCs) programs. The chapters in this book cover topics such as conjugations to endogenous cysteine residues; click chemistry conjugations; antibody conjugations via glycosyl remodeling; analysis of ADCs by native mass spectrometry; characterization of ADCs by capillary electrophoresis; LC/MS methods for studying lysosomal ADC catabolism; and determination of ADC concentration by ligand-binding assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Antibody-Drug Conjugates: Methods and Protocols is a valuable resource that aims to lower the “activation barrier” when undertaking a new discipline, and provides a “toolbox” for the next generation of ADC scientists.


ADCs Conjugation procedures Bioanalysis Purification Production

Editors and affiliations

  • L. Nathan Tumey
    • 1
  1. 1.Department of Pharmacy and Pharmaceutical SciencesBinghamton UniversityBinghamtonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9928-6
  • Online ISBN 978-1-4939-9929-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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