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Chimeric RNA

Methods and Protocols

  • Hui Li
  • Justin Elfman
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2079)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Identification and Characterization

    1. Front Matter
      Pages 1-1
    2. Neetha Nanoth Vellichirammal, Abrar Albahrani, You Li, Chittibabu Guda
      Pages 13-25
    3. Jin Zhang, Christopher A. Maher
      Pages 41-68
  3. Experimental Validation

    1. Front Matter
      Pages 81-81
    2. Justin Elfman, Hui Li
      Pages 83-94
    3. Félix LaRoche-Johnston, Caroline Monat, Benoit Cousineau
      Pages 95-107
    4. Jianzhu Zhao, Jun Tang, Justin Elfman, Hui Li
      Pages 109-116
  4. Functional and Mechanistic Studies

    1. Front Matter
      Pages 141-141
    2. Fujun Qin, Xinrui Shi, Hui Li
      Pages 143-154
    3. Xinrui Shi, Fujun Qin, Hui Li
      Pages 177-186
    4. Sachin Kumar Gupta, Jocelyn Duen-Ya Jea, Laising Yen
      Pages 187-207
  5. Translational Applications

    1. Front Matter
      Pages 209-209
    2. Xiurong Ke, Xiao Xiong, Yusheng Lin, Hao Zhang
      Pages 211-218
    3. Lisa M. Riedmayr
      Pages 219-232
  6. Back Matter
    Pages 259-262

About this book

Introduction

This book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes. Beginning with identification and characterization through bioinformatics tools, the book continues with experimental validation using RT-PCR, hybridization methods for RNA, and the use of mass spectrometry data for fusion protein validation, as well as functional and mechanistic studies of chimeric RNAs and translational applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Chimeric RNA: Methods and Protocols is an ideal guide to this expansive field and an inspiration to researchers seeking to further our knowledge of these remarkable features of the transcriptome.

Keywords

Gene fusion Transcription-mediated fusions Fusion transcripts Bioinformatics Translational applications

Editors and affiliations

  • Hui Li
    • 1
  • Justin Elfman
    • 2
  1. 1.Department of Paathology, School of Medicine, University of Virginia, Charlottesville, VA, USADepartment of Biochemistry and Molecular Genetics, School of Medicine, University of VirginiaCharlottesvilleUSA
  2. 2.Department of Biochemistry and Molecular GeneticsUniversity of VirginiaCharlottesvilleUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-9904-0
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9903-3
  • Online ISBN 978-1-4939-9904-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site