Histidine Phosphorylation

Methods and Protocols

  • Claire E. Eyers

Part of the Methods in Molecular Biology book series (MIMB, volume 2077)

Table of contents

  1. Front Matter
    Pages i-x
  2. Jonathan T. Fischer, Ilana Heckler, Elizabeth M. Boon
    Pages 37-49
  3. Alice K. M. Clubbs Coldron, Dominic P. Byrne, Patrick A. Eyers
    Pages 63-81
  4. Lauren J. Tomlinson, Alice K. M. Clubbs Coldron, Patrick A. Eyers, Claire E. Eyers
    Pages 83-91
  5. Brandon S. McCullough, Amy M. Barrios
    Pages 109-120
  6. Laura Miguel-Romero, Cristina Mideros-Mora, Alberto Marina, Patricia Casino
    Pages 121-140
  7. Kimberly A. Kowallis, Samuel W. Duvall, Wei Zhao, W. Seth Childers
    Pages 141-163
  8. Aleksandra E. Bury, Klaas J. Hellingwerf
    Pages 165-180
  9. Andrew R. Jones, Oscar Martin Camacho
    Pages 237-250
  10. Back Matter
    Pages 251-256

About this book


This volume details the current understanding of roles and regulation on histidine phosphorylation, describing methods for the characterization of protein phosphorylation on histidine. Chapters guide readers through in vitro systems, cell-based systems, comprehensive background review articles on histidine kinases and phosphatases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Histidine Phosphorylation: Methods and Protocols aims to ensure successful results in the further study of this rapidly growing field. 


histidine kinase substrates pHis phosphorylated proteins mammalian cell signaling histidine phosphorylation

Editors and affiliations

  • Claire E. Eyers
    • 1
  1. 1.Department of Biochemistry, Centre for Proteome Research Institute of Integrative BiologyUniversity of LiverpoolLiverpoolUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9883-8
  • Online ISBN 978-1-4939-9884-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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