Horizontal Gene Transfer

Methods and Protocols

  • Fernando de la Cruz

Part of the Methods in Molecular Biology book series (MIMB, volume 2075)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Methods for the Detection and Isolation of Mobile Genetic Elements

    1. Front Matter
      Pages 1-1
    2. Kaitlin A. Tagg, Carola Venturini, Muhammad Kamruzzaman, Andrew N. Ginn, Sally R. Partridge
      Pages 3-20
    3. Ana R. Freitas, Carla Novais, Luísa Peixe, Teresa M. Coque
      Pages 21-38
    4. Khald Blau, Sven Jechalke, Kornelia Smalla
      Pages 39-60
    5. Catherine Guynet, Phan Thai Nguyen Le, Michael Chandler, Bao Ton-Hoang
      Pages 81-90
  3. Methods to Quantify and Characterize Horizontal Gene Transfer

    1. Front Matter
      Pages 91-91
    2. Jorge Rodriguez-Grande, Raul Fernandez-Lopez
      Pages 93-98
    3. Carolina Palencia-Gandara, Raul Ruiz, Raul Fernandez-Lopez
      Pages 99-110
    4. Carla Novais, Ana R. Freitas, Ricardo León-Sampedro, Luísa Peixe, Teresa M. Coque
      Pages 111-122
    5. Ryan R. Clark, Todd A. Gray, Keith M. Derbyshire
      Pages 123-134
    6. Elena Cabezón, Ignacio Arechaga
      Pages 135-143
    7. Catherine Guynet, Emilien Nicolas, Bao Ton-Hoang, Jean-Yves Bouet, Bernard Hallet
      Pages 157-177
    8. Yoko Komiyama, Sumio Maeda
      Pages 179-187
    9. Claire Vit, Céline Loot, José Antonio Escudero, Aleksandra Nivina, Didier Mazel
      Pages 189-208
    10. Alba Blesa, José Berenguer
      Pages 209-221
    11. Damián Lobato-Márquez
      Pages 223-233
  4. OMICs, Bioinformatics, Mathematical Modeling, and Technological Applications of HGT

    1. Front Matter
      Pages 251-251
    2. Patrick Denis Browne, Witold Kot, Tue Sparholt Jørgensen, Lars Hestbjerg Hansen
      Pages 253-264
    3. Jean Cury, Sophie S. Abby, Olivia Doppelt-Azeroual, Bertrand Néron, Eduardo P. C. Rocha
      Pages 265-283
    4. M. Pilar Garcillán-Barcia, Santiago Redondo-Salvo, Luis Vielva, Fernando de la Cruz
      Pages 295-308
    5. Laura Villa, Alessandra Carattoli
      Pages 309-321
    6. María de Toro, Val F. Lanza, Luis Vielva, Santiago Redondo-Salvo, Fernando de la Cruz
      Pages 323-339
    7. Miguel D. Fernández-de-Bobadilla, Teresa M. Coque, Val F. Lanza
      Pages 341-353
    8. Luis Delaye, Carlos Vargas, Amparo Latorre, Andrés Moya
      Pages 355-369
    9. Javier DelaFuente, Jeronimo Rodriguez-Beltran, Alvaro San Millan
      Pages 371-382
    10. Tomás Aparicio, Víctor de Lorenzo, Esteban Martínez-García
      Pages 383-398
  5. Back Matter
    Pages 411-413

About this book


This book focuses on technologies used to study horizontal gene transfer (HGT) in prokaryotes. Beginning with a section on the detection and isolation of mobile genetic elements (MGEs), the volume continues with sections concentrating on the analysis of conjugation, transformation, and transduction in HGT as well as a series of methods to analyze the adaptation and evolution of MGEs, with special attention paid to bioinformatics tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Horizontal Gene Transfer: Methods and Protocols serves as an ideal guide to the further study of this pervasive, all-important mechanism of genetic originality.


MGEs HGT Prokaryotes Microbial ecosystems Bacterial populations Mobile genetic elements

Editors and affiliations

  • Fernando de la Cruz
    • 1
  1. 1.Instituto de Biomedicina y Biotecnología de CantabriaUniversidad de Cantabria-CSICSantanderSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9876-0
  • Online ISBN 978-1-4939-9877-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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