Toxoplasma gondii

Methods and Protocols

  • Christopher J. Tonkin

Part of the Methods in Molecular Biology book series (MIMB, volume 2071)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Chunlei Su, Jitender P. Dubey
    Pages 49-80
  3. Federica Piro, Vern B. Carruthers, Manlio Di Cristina
    Pages 117-123
  4. Catherine Suarez, Melissa B. Lodoen, Maryse Lebrun
    Pages 143-155
  5. Kevin M. Brown, L. David Sibley, Sebastian Lourido
    Pages 157-169
  6. Emily Shortt, Sebastian Lourido
    Pages 171-186
  7. Stephen A. Vella, Abigail Calixto, Beejan Asady, Zhu-Hong Li, Silvia N. J. Moreno
    Pages 187-207
  8. Georgios Pavlou, Isabelle Tardieux
    Pages 209-220
  9. Marco Biddau, Jana Ovciarikova, Lilach Sheiner
    Pages 221-243
  10. Esther Rajendran, Kiaran Kirk, Giel G. van Dooren
    Pages 245-268
  11. Joshua Mayoral, Manlio Di Cristina, Vern B. Carruthers, Louis M. Weiss
    Pages 269-282
  12. Carla M. Cabral, Emily F. Merritt, Anita A. Koshy
    Pages 283-295
  13. Justine R. Smith, Liam M. Ashander, Yuefang Ma, Elise Rochet, João M. Furtado
    Pages 297-321
  14. Peter J. Bradley, Shima Rayatpisheh, James A. Wohlschlegel, Santhosh M. Nadipuram
    Pages 323-346
  15. Debanjan Mukhopadhyay, Jeroen P. J. Saeij
    Pages 347-370
  16. Catalina Alvarez, Ana Claudia Campos, Jonathan C. Howard, Joana Loureiro, Urs Benedikt Müller, Ana Lina Rodrigues
    Pages 371-409
  17. Daniel Fisch, Artur Yakimovich, Barbara Clough, Jason Mercer, Eva-Maria Frickel
    Pages 411-433
  18. Elizabeth F. B. King, Simon A. Cobbold, Alessandro D. Uboldi, Christopher J. Tonkin, Malcolm J. McConville
    Pages 435-452
  19. Back Matter
    Pages 469-470

About this book


This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii strains; assessing rhoptry secretion in T. gondii; plate-based quantification of stimulated Toxoplasma egress; methods to study ocular toxoplasmosis; and metabolic analysis of Toxoplasma gondii tachyzoites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Toxoplasma gondii: Methods and Protocols is a valuable resource for students or groups starting off in the field, as well as laboratories interested in implementing the latest techniques described in the book.


bradyzoites mouse model Apical organelles Host cell Egress tissue culture

Editors and affiliations

  • Christopher J. Tonkin
    • 1
  1. 1.Division of Infectious Disease and Immune DefenseThe Walter and Eliza Hall Institute of Medical ResearchMelbourneAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9856-2
  • Online ISBN 978-1-4939-9857-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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