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Genotype Phenotype Coupling

Methods and Protocols

  • Stefan Zielonka
  • Simon Krah
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2070)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Valentina Kalichuk, Stanimir Kambarev, Ghislaine Béhar, Benjamin Chalopin, Axelle Renodon-Cornière, Barbara Mouratou et al.
    Pages 19-41
  3. Valentin Jakob, Saskia Helmsing, Michael Hust, Martin Empting
    Pages 95-113
  4. John Steven, Obinna C. Ubah, Magdalena Buschhaus, Marina Kovaleva, Laura Ferguson, Andrew J. Porter et al.
    Pages 115-142
  5. Esther V. Wenzel, Kristian D. R. Roth, Giulio Russo, Viola Fühner, Saskia Helmsing, André Frenzel et al.
    Pages 143-155
  6. Kathryn M. Frietze, Susan B. Core, Alexandria Linville, Bryce Chackerian, David S. Peabody
    Pages 157-171
  7. Lukas Roth, Simon Krah, Janina Klemm, Ralf Günther, Lars Toleikis, Michael Busch et al.
    Pages 173-189
  8. Doreen Könning, Stefan Zielonka, Anna Kaempffe, Sebastian Jäger, Harald Kolmar, Christian Schröter
    Pages 191-209
  9. Steffen C. Hinz, Adrian Elter, Julius Grzeschik, Jan Habermann, Bastian Becker, Harald Kolmar
    Pages 211-222
  10. Flávio Sádio, Gerhard Stadlmayr, Katja Eibensteiner, Katharina Stadlbauer, Florian Rüker, Gordana Wozniak-Knopp
    Pages 223-248
  11. Anna Kaempffe, Sebastian Jäger, Doreen Könning, Harald Kolmar, Christian Schröter
    Pages 249-266
  12. Sebastian Jäger, Simon Krah, Doreen Könning, Janis Rosskopf, Stephan Dickgiesser, Nicolas Rasche et al.
    Pages 267-287
  13. Jan P. Bogen, Julius Grzeschik, Simon Krah, Stefan Zielonka, Harald Kolmar
    Pages 289-302
  14. Lawrence A. Stern, Patrick S. Lown, Benjamin J. Hackel
    Pages 303-320
  15. Simon Krah, Ralf Günther, Stefan Becker, Stefan Zielonka, Laura Rhiel
    Pages 335-349
  16. Zachary R. Crook, Gregory P. Sevilla, Andrew J. Mhyre, James M. Olson
    Pages 363-396
  17. Annalee W. Nguyen, Kevin Le, Jennifer A. Maynard
    Pages 397-422
  18. Juliet Rashidian, Joshua Lloyd
    Pages 423-441
  19. Back Matter
    Pages 443-445

About this book

Introduction

This volume aims at providing state-of-the-art protocols detailing ribosome display, cDNA display, phage display, yeast surface display, and mammalian display. Chapters guide readers through methods and protocols on in vitro methods over prokaryotic display systems, lower eukaryotes, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of all necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Genotype Phenotype Coupling aims to provide an overview of current technologies in this exciting and continuously evolving field. 


Keywords

phage-assisted Continuous Evolution (PACE) Phage Display SELEX genotype phenotype coupling Aptamers

Editors and affiliations

  • Stefan Zielonka
    • 1
  • Simon Krah
    • 2
  1. 1.Protein Engineering and Antibody TechnologiesMerck KGaADarmstadtGermany
  2. 2.Protein Engineering and Antibody TechnologiesMerck KGaADarmstadtGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-9853-1
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9852-4
  • Online ISBN 978-1-4939-9853-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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