Diabetic Nephropathy

Methods and Protocols

  • Luigi Gnudi
  • David A. Long

Part of the Methods in Molecular Biology book series (MIMB, volume 2067)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Manpreet K. Sagoo, Luigi Gnudi
      Pages 3-7
  3. Experimental Models to Study Diabetic Nephropathy

  4. Techniques to Image the Diabetic Kidney

    1. Front Matter
      Pages 61-61
    2. Camillo Carrara, Mauro Abbate, Sara Conti, Daniela Rottoli, Paola Rizzo, Gianfranco Marchetti
      Pages 63-87
    3. Daniyal J. Jafree, David A. Long, Peter J. Scambler, Dale Moulding
      Pages 103-126
  5. Assessing the Function and Pathophysiological Properties of the Diabetic Kidney

    1. Front Matter
      Pages 127-127
    2. Cristina Daniele, Daniela Nardozi, Angelo Torelli, Arif ul Maula Khan, Norbert Gretz
      Pages 129-137
    3. Abigail C. Lay, Richard J. Coward
      Pages 139-143
    4. Raina D. Ramnath, Simon C. Satchell
      Pages 145-151
    5. Khurrum Shahzad, Sanchita Ghosh, Akash Mathew, Berend Isermann
      Pages 153-173
    6. Karina Barreiro, Tobias B. Huber, Harry Holthofer
      Pages 175-188
    7. Eleftherios Siamantouras, Claire E. Hills, Kuo-Kang Liu, Paul E. Squires
      Pages 189-201
  6. Transcriptome, Metabolic, and Proteomic Profiling of the Diabetic Kidney

    1. Front Matter
      Pages 203-203
    2. Laura J. Smyth, Katie Kerr, Seamus Duffy, Jill Kilner, Amy Jayne McKnight
      Pages 205-240
    3. Marisa Canadas-Garre, Laura J. Smyth, Kerry Anderson, Katie Kerr, Amy Jayne McKnight
      Pages 241-275
    4. Lucy Jade Newbury, Alexa Wonnacott, Kate Simpson, Timothy Bowen, Donald Fraser
      Pages 277-285
  7. New Approaches: Gene Editing and Stem Cells to Study Diabetic Kidney Disease

    1. Front Matter
      Pages 307-307
    2. Ioannis Bantounas, Edina Silajdžić, Adrian S. Woolf, Susan J. Kimber
      Pages 309-322
    3. Nuria Perretta-Tejedor, Grace Freke, Marian Seda, David A. Long, Dagan Jenkins
      Pages 323-340 Open Access
  8. Back Matter
    Pages 341-342

About this book


This book provides a toolkit of novel research approaches for investigators to study diabetic nephropathy, including critical experimental models from the fly to the fish, cells in culture, and in vivo mammalian approaches. The collection also explores powerful techniques to image the kidney, such as traditional histological techniques as well as electron, confocal, and two-photon microscopy, pathophysiology of the diabetic kidney, and gene editing and regenerative medicine. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Diabetic Nephropathy: Methods and Protocols seeks to foster new research directions and inspire ideas to enhance our understanding of diabetic nephropathy and to develop treatments for this condition.


Diabetes mellitus Renal failure Kidney imaging Pathophysiology Regenerative medicine Diabetic kidney disease Experimental models.

Editors and affiliations

  • Luigi Gnudi
    • 1
  • David A. Long
    • 2
  1. 1.Faculty of Life Sciences & Medicine School of Cardiovascular Medicine & Sciences, British Heart Foundation Centre of Research ExcellenceKing’s College LondonLondonUK
  2. 2.Developmental Biology and Cancer Programme, UCL Great Ormond Street Institute of Child HealthUniversity College LondonLondonUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9840-1
  • Online ISBN 978-1-4939-9841-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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