Single Cell Metabolism

Methods and Protocols

  • Bindesh Shrestha

Part of the Methods in Molecular Biology book series (MIMB, volume 2064)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Zhenwei Wei, Xiaochao Zhang, Xingyu Si, Xiaoyun Gong, Sichun Zhang, Xinrong Zhang
    Pages 31-59
  3. Dhaka Ram Bhandari, Giulia Coliva, Maria Fedorova, Bernhard Spengler
    Pages 103-111
  4. Jasmin Krismer, Jens Sobek, Robert F. Steinhoff, Rolf Brönnimann, Martin Pabst, Renato Zenobi
    Pages 113-124
  5. Bo Yang, Tina Tsui, Richard M. Caprioli, Jeremy L. Norris
    Pages 125-134
  6. Phuong Le, Shweta Chitoor, Chunlai Tu, Sung Jun Lim, Andrew M. Smith
    Pages 147-158
  7. Christina R. Ferreira, Valentina Pirro, Alan K. Jarmusch, Clint M. Alfaro, R. Graham Cooks
    Pages 159-179
  8. Anthony Midey, Hernando Olivos, Bindesh Shrestha
    Pages 181-190
  9. Back Matter
    Pages 225-226

About this book


This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful I series format, the chapters consist of  introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field. 


mass spectrometry metabolomics MALDI MS imaging oocytes cellular metabolites lipidomic analysis

Editors and affiliations

  • Bindesh Shrestha
    • 1
  1. 1.Waters CorporationMilfordUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9829-6
  • Online ISBN 978-1-4939-9831-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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