The Eukaryotic RNA Exosome

Methods and Protocols

  • John LaCava
  • Štěpánka Vaňáčová

Part of the Methods in Molecular Biology book series (MIMB, volume 2062)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Introduction to the RNA Exosome in Biomedicine

    1. Front Matter
      Pages 1-1
    2. Milo B. Fasken, Derrick J. Morton, Emily G. Kuiper, Stephanie K. Jones, Sara W. Leung, Anita H. Corbett
      Pages 3-33
  3. Prokaryotic RNases and Exosomes

    1. Front Matter
      Pages 35-35
    2. Sandra C. Viegas, Rute G. Matos, Cecília M. Arraiano
      Pages 37-46
    3. Rute G. Matos, Sandra C. Viegas, Cecília M. Arraiano
      Pages 47-61
    4. Elena Evguenieva-Hackenberg, A. Susann Gauernack, Linlin Hou, Gabriele Klug
      Pages 63-79
  4. Analyzing Exosome Substrates

    1. Front Matter
      Pages 81-81
    2. Cristina Cruz, Jonathan Houseley
      Pages 83-103 Open Access
    3. Clémentine Delan-Forino, David Tollervey
      Pages 105-126 Open Access
    4. Hélène Scheer, Caroline De Almeida, Natalia Sikorska, Sandrine Koechler, Dominique Gagliardi, Hélène Zuber
      Pages 147-167
    5. Veronika A. Herzog, Nina Fasching, Stefan L. Ameres
      Pages 169-189
    6. Alexandra Lusser, Catherina Gasser, Lukas Trixl, Paolo Piatti, Isabel Delazer, Dietmar Rieder et al.
      Pages 191-211
  5. Exosomes and Their Supramolecular Functional Interactions

    1. Front Matter
      Pages 213-213
    2. Cornelia Kilchert
      Pages 215-235
    3. Cornelia Kilchert, Svenja Hester, Alfredo Castello, Shabaz Mohammed, Lidia Vasiljeva
      Pages 255-276
    4. Kinga Winczura, Michal Domanski, John LaCava
      Pages 291-325
    5. Alexandra Zinoviev, Christopher U. T. Hellen, Tatyana V. Pestova
      Pages 327-354
  6. Recombinant Expression Strategies and Structural Characterization of Exosomes

    1. Front Matter
      Pages 355-355
    2. Jun-Jie Liu, Hong-Wei Wang
      Pages 401-415
    3. Eva-Maria Weick, John C. Zinder, Christopher D. Lima
      Pages 417-425
    4. Kurt Januszyk, Christopher D. Lima
      Pages 449-465
    5. Kurt Januszyk, Eva-Maria Weick, Christopher D. Lima
      Pages 467-489
  7. Back Matter
    Pages 515-517

About this book


This volume provides a cross-section of RNA exosome research protocols, applied to a diversity of model organisms. Chapters guide readers through methods that e.g. delineate eukaryotic exosomes’ origins in prokaryotes, probe its RNA substrates, adapter complexes, and macromolecular interaction of networks, and establish critical structure-function relationships. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Eukaryotic RNA Exosome: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Authoritative and cutting-edge, Th e Eukaryotic RNA Exosome: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Chapters 5 and 6 are available open access under a Creative Commons Attribution 4.0 International License via


RNA-seq analysis cDNA library preparation yeast exosome substrates RNAi Archeal exosomes

Editors and affiliations

  • John LaCava
    • 1
  • Štěpánka Vaňáčová
    • 2
  1. 1.Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY, USAEuropean Research Institute for the Biology of Ageing, University Medical Center GroningenGroningenThe Netherlands
  2. 2.Central European Institute of Technology (CEITEC)Masaryk UniversityBrnoCzech Republic

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9821-0
  • Online ISBN 978-1-4939-9822-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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