Herpes Simplex Virus

Methods and Protocols

  • Russell J. Diefenbach
  • Cornel Fraefel

Part of the Methods in Molecular Biology book series (MIMB, volume 2060)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Christopher E. Denes, Roger D. Everett, Russell J. Diefenbach
    Pages 1-30
  3. Kerrie J. Sandgren, Naomi R. Truong, Jacinta B. Smith, Kirstie Bertram, Anthony L. Cunningham
    Pages 31-56
  4. Sereina O. Sutter, Peggy Marconi, Anita F. Meier
    Pages 57-72
  5. William F. Goins, Shaohua Huang, Bonnie Hall, Marco Marzulli, Justus B. Cohen, Joseph C. Glorioso
    Pages 73-90
  6. Cornel Fraefel, Alberto L. Epstein
    Pages 91-109
  7. Anita F. Meier, Andrea S. Laimbacher
    Pages 111-130
  8. Laura Menotti, Valerio Leoni, Valentina Gatta, Biljana Petrovic, Andrea Vannini, Simona Pepe et al.
    Pages 131-151
  9. Andrea Vannini, Biljana Petrovic, Valentina Gatta, Valerio Leoni, Simona Pepe, Laura Menotti et al.
    Pages 153-168
  10. Thilaga Velusamy, Anjali Gowripalan, David C. Tscharke
    Pages 169-183
  11. Camille Cohen, Armelle Corpet, Mohamed Ali Maroui, Franceline Juillard, Patrick Lomonte
    Pages 185-197
  12. Mackenzie M. Shipley, Molly M. Rathbun, Moriah L. Szpara
    Pages 199-217
  13. Andreas Sauerbrei, Kathrin Bohn-Wippert
    Pages 241-261
  14. Hui-Lan Hu, Kalanghad Puthankalam Srinivas, Ian Mohr, Tony T. Huang, Angus C. Wilson
    Pages 263-277
  15. Bita Khadivjam, Nabil El Bilali, Roger Lippé
    Pages 289-303
  16. Raquel Bello-Morales, José Antonio López-Guerrero
    Pages 305-317
  17. Tri Komala Sari, Katrina A. Gianopulos, Anthony V. Nicola
    Pages 319-326
  18. Monica Miranda-Saksena, Ross A. Boadle, Anthony L. Cunningham
    Pages 343-354
  19. Ellen M. White, Samuel D. Stampfer, Ekaterina E. Heldwein
    Pages 377-393
  20. Kevin Danastas, Anthony L. Cunningham, Monica Miranda-Saksena
    Pages 409-418
  21. Joshua O. Marshak, Lichun Dong, David M. Koelle
    Pages 429-454
  22. Back Matter
    Pages 455-457

About this book


This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Herpes Simplex Virus: Methods and Protocols, Second Edition is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and/or cellular-based approaches to study HSV. 


Nucleocapsid DNA Bacterial Artificial Chromosomes (BACs) Latent/Lytic Cycles In Vivo Cryo-EM CRISPR-Cas9

Editors and affiliations

  • Russell J. Diefenbach
    • 1
  • Cornel Fraefel
    • 2
  1. 1.Department of Biomedical SciencesMacquarie UniversitySydneyAustralia
  2. 2.Institute of VirologyUniversity of ZurichZürichSwitzerland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9813-5
  • Online ISBN 978-1-4939-9814-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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