Nitrogen Metabolism in Plants

Methods and Protocols

  • Kapuganti Jagadis  Gupta

Part of the Methods in Molecular Biology book series (MIMB, volume 2057)

Table of contents

  1. Front Matter
    Pages i-x
  2. Reddy Kishorekumar, Mallesham Bulle, Aakanksha Wany, Kapuganti Jagadis Gupta
    Pages 1-13
  3. Aakanksha Wany, Pradeep Kumar Pathak, Kapuganti Jagadis Gupta
    Pages 15-26
  4. Mallesham Bulle, Reddy Kishorekumar, Pradeep K. Pathak, Aakanksha Wany, Kapuganti Jagadis Gupta
    Pages 27-35
  5. Paulo T. Mioto, Alejandra Matiz, Luciano Freschi, Francisco J. Corpas
    Pages 37-43
  6. Martina Janků, Tereza Tichá, Lenka Luhová, Marek Petřivalský
    Pages 45-59
  7. Mallesham Bulle, Reddy Kishorekumar, Aakanksha Wany, Kapuganti Jagadis Gupta
    Pages 61-69
  8. Alexander T. Eprintsev, Natalia V. Selivanova, Abir U. Igamberdiev
    Pages 71-78
  9. Namrata Singh, Pooja Singh, Pradeep Kumar Pathak, Kapuganti Jagadis Gupta
    Pages 79-92
  10. Reena Arora, Pooja Singh, Aprajita Kumari, Pradeep Kumar Pathak, Kapuganti Jagadis Gupta
    Pages 93-102
  11. Pratiksha Singh, Rajesh Kumar Singh, Qi-Qi Song, Hai-Bi Li, Li-Tao Yang, Yang-Rui Li
    Pages 103-112
  12. Tirthankar Bandyopadhyay, Manoj Prasad
    Pages 113-118
  13. Engil I. P. Pereira, Marcelo C. M. Teixeira Filho
    Pages 145-153
  14. Eman Elagamey, Kanika Narula, Niranjan Chakraborty, Subhra Chakraborty
    Pages 155-172
  15. Back Matter
    Pages 173-178

About this book


This volume explores several different aspects of nitrogen metabolism, ranging from nitrogen uptake to assimilation. The chapters in this book cover topics such as measurement of activities of enzymes involved in nitrogen metabolism (i.e., nitrate reductase); measurement of nitric oxide, nitrite and ways to detect the N content in plants and microbes; characterization of root nodule bacteria (RNB); and techniques to perform proteomics of Extracellular Matrix (ECM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Nitrogen Metabolism in Plants: Methods and Protocols is a valuable tool for novice and expert researchers who are interested in learning more about this evolving field.


nitrogen assimilation enzymes nitrogen symbiosis AccQ•Tag Ultra Performance Liquid Chromatography Arabidopsis

Editors and affiliations

  • Kapuganti Jagadis  Gupta
    • 1
  1. 1.National Institute of Plant Genome ResearchNew DelhiIndia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9789-3
  • Online ISBN 978-1-4939-9790-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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