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Trinucleotide Repeats

Methods and Protocols

  • Guy-Franck Richard
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2056)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Stéphanie Tomé, Geneviève Gourdon
    Pages 11-23
  3. Gregory M. Williams, Athena K. Petrides, Lata Balakrishnan, Jennifer A. Surtees
    Pages 25-68
  4. Erica J. Polleys, Catherine H. Freudenreich
    Pages 83-101
  5. Rujuta Yashodhan Gadgil, S. Dean Rider Jr., Todd Lewis, Joanna Barthelemy, Michael Leffak
    Pages 121-136
  6. Artem V. Kononenko, Thomas Ebersole, Sergei M. Mirkin
    Pages 137-150
  7. Gregory M. Williams, Robert S. Lahue
    Pages 151-172
  8. Xiaonan Zhao, Huiyan Lu, Pradeep K. Dagur, Karen Usdin
    Pages 173-186
  9. Remco T. P. van Cruchten, Derick G. Wansink
    Pages 187-202
  10. Arnaud F. Klein, Ludovic Arandel, Joelle Marie, Denis Furling
    Pages 203-215
  11. Sandra O. Braz, Diana M. Dinca, Geneviève Gourdon, Mário Gomes-Pereira
    Pages 217-240
  12. Cinzia Cinesi, Bin Yang, Vincent Dion
    Pages 255-268
  13. Magdalena Dabrowska, Marta Olejniczak
    Pages 269-284
  14. Back Matter
    Pages 285-286

About this book

Introduction

This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats.  Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as a valuable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study.

Keywords

Genetic screens Pathologies Saccharomyces cerevisiae Human cells Experimental systems Gene therapy CRISPR-Cas Human disorders Genetic mutations

Editors and affiliations

  1. 1.Department Genomes & GeneticsInstitut PasteurParisFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-9784-8
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9783-1
  • Online ISBN 978-1-4939-9784-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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