Biomarkers for Immunotherapy of Cancer

Methods and Protocols

  • Magdalena Thurin
  • Alessandra Cesano
  • Francesco M. Marincola

Part of the Methods in Molecular Biology book series (MIMB, volume 2055)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Scientific Questions and the Status of Inhibitory Receptors for Immunotherapy

    1. Front Matter
      Pages 1-1
    2. Alessandra Cesano, Francesco M. Marincola, Magdalena Thurin
      Pages 3-21
    3. Diwakar Davar, Hassane M. Zarour
      Pages 23-60
  3. Mutational and Germline Profiling

    1. Front Matter
      Pages 61-61
    2. Russell Bonneville, Melanie A. Krook, Hui-Zi Chen, Amy Smith, Eric Samorodnitsky, Michele R. Wing et al.
      Pages 119-132
    3. Giuseppe Palmieri, Milena Casula, Antonella Manca, Grazia Palomba, Maria Cristina Sini, Valentina Doneddu et al.
      Pages 133-154
    4. Mahrukh M. Syeda, Jennifer M. Wiggins, Broderick Corless, Cindy Spittle, George Karlin-Neumann, David Polsky
      Pages 155-180
  4. Epigenetic Profiling

    1. Front Matter
      Pages 201-201
    2. Erin M. Taylor, Brian Koss, Lauren E. Davis, Alan J. Tackett
      Pages 213-228
  5. Functional Genomic Profiling

    1. Front Matter
      Pages 229-229
    2. Ena Wang, Davide Bedognetti, Francesco M. Marincola
      Pages 231-244
    3. C. C. Smith, L. M. Bixby, K. L. Miller, S. R. Selitsky, D. S. Bortone, K. A. Hoadley et al.
      Pages 245-272
    4. Sarah Warren, Patrick Danaher, Afshin Mashadi-Hossein, Lynell Skewis, Brett Wallden, Sean Ferree et al.
      Pages 273-300
    5. Vitaly Balan, Jianbin Wang
      Pages 301-322
  6. Phenotyping of Tumor and Immune Cells

    1. Front Matter
      Pages 323-323
    2. Bita Sahaf, Adeeb Rahman, Holden T. Maecker, Sean C. Bendall
      Pages 351-368
    3. Takahiro Yamazaki, Aitziber Buqué, Marissa Rybstein, Jonathan Chen, Ai Sato, Lorenzo Galluzzi
      Pages 433-452
  7. Tissue Imaging Methods

    1. Front Matter
      Pages 453-453
    2. Sanjay S. Patel, Scott J. Rodig
      Pages 455-465
    3. Alejandro Francisco-Cruz, Edwin Roger Parra, Michael T. Tetzlaff, Ignacio I. Wistuba
      Pages 467-495
    4. Jennifer Eng, Guillaume Thibault, Shiuh-Wen Luoh, Joe W. Gray, Young Hwan Chang, Koei Chin
      Pages 521-562
  8. Microbiome

    1. Front Matter
      Pages 593-593
    2. Manoj Kumar, Parul Singh, Selvasankar Murugesan, Marie Vetizou, John McCulloch, Jonathan H. Badger et al.
      Pages 595-638
  9. System Biology for Multi-platform Data Integration

    1. Front Matter
      Pages 639-639
    2. Alida Palmisano, Julia Krushkal, Ming-Chung Li, Jianwen Fang, Dmitriy Sonkin, George Wright et al.
      Pages 649-678
  10. Regulatory Perspective

    1. Front Matter
      Pages 699-699
    2. You Li, Janaki Veeraraghavan, Reena Philip
      Pages 701-716
  11. Takahiro Yamazaki, Aitziber Buqué, Marissa Rybstein, Jonathan Chen, Ai Sato, Lorenzo Galluzzi
    Pages C1-C1
  12. Back Matter
    Pages 717-733

About this book


This book provides the immune oncology (IO) community with a deeper understanding of the scope of the biomarker methods to potentially improve the outcome from immunotherapy. The editors secured the input from experts in the field dedicated to translating scientific research from bench to bedside was submitted. The book provides not only details about the technical, standardization and interpretation aspects of the methods but also introduces the reader to the background information and scientific justification for selected biomarkers and assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Immuno-Oncology FISH/RNA ISH CRISPR/Cas9 HLA/KIR matching EliSPOT

Editors and affiliations

  • Magdalena Thurin
    • 1
  • Alessandra Cesano
    • 2
  • Francesco M. Marincola
    • 3
  1. 1.NIHNational Cancer InstituteBethesdaUSA
  2. 2.Nanostring Technologies, Seattle, WA, USAESSA PharmaSouth San FranciscoUSA
  3. 3.Refuge BiotechnologiesMenlo ParkUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9772-5
  • Online ISBN 978-1-4939-9773-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals
Oncology & Hematology
Consumer Packaged Goods