Advertisement

© 2020

Fundamental Approaches to Screen Abnormalities in Drosophila

  • Monalisa Mishra
Book

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-vii
  2. Subhashree Priyadarsini, Sumit Mukherjee, Monalisa Mishra
    Pages 1-13
  3. Janmejaya Bag, Monalisa Mishra
    Pages 31-38
  4. Subhashree Priyadarsini, Sumit Mukherjee, Monalisa Mishra
    Pages 51-64
  5. Madhavi Dubey, Kumari Pragati Nanda, Hena Firdaus
    Pages 65-76
  6. Gyanaseni Dhar, Monalisa Mishra
    Pages 87-96
  7. Ronnaly Rout, Srirupa Basu, Monalisa Mishra
    Pages 97-109
  8. Nibedita Nayak, Monalisa Mishra
    Pages 111-122
  9. Nibedita Nayak, Monalisa Mishra
    Pages 123-134
  10. Sumit Mukherjee, Monalisa Mishra
    Pages 135-149
  11. Manish Pandey, Sakshi Bansal, Geetanjali Chawla
    Pages 169-188
  12. Abhinandan Patnaik, Monalisa Mishra
    Pages 189-198
  13. Gyanaseni Dhar, Sumit Mukherjee, Nibedita Nayak, Swetapadma Sahu, Janmejaya Bag, Ronnaly Rout et al.
    Pages 223-251
  14. Swetapadma Sahu, Gyanaseni Dhar, Monalisa Mishra
    Pages 253-265

About this book

Introduction

This volume includes in-depth, hands-on protocols for detecting various developmental defects in Drosophila. It provides cutting-edge methods for maintaining Drosophila under laboratory conditions to perform various experiments, and for dissecting various imaginal discs of the larvae. Further, biochemical protocols for estimating the levels of different metabolites, reactive oxygen species, and fat-sensitive pathways are discussed, and various staining and behavior techniques for fat detection are provided.

The book explains how various fluorescent dyes and the comet assay can be used to identify DNA damage, and elaborates on the analysis of the eye, antennae, imaginal disc, gut and muscle damage under bright field and fluorescent microscopes. It covers the analysis of hemolymph using Giemsa staining; determining the functionality of channel proteins, eye and mechanosensory organs in adults and larvae using various behavioral assays; and metal detection and structural analysis for various tissues using SEM. In closing, it addresses the analysis of Drosophila’s eye and head via paraffin section; measurement of reactive oxygen species from various tissues via FACS; and the CRISPER technique for gene editing and analysis of micro RNA mutations.

Keywords

Drosophila Histological techniques Biochemical methods Behavioral defect Microscopy analysis

Editors and affiliations

  • Monalisa Mishra
    • 1
  1. 1.Neural Developmental Biology Lab, Department of Life ScienceNational Institute of Technology RourkelaRourkelaIndia

About the editors

Dr. Monalisa Mishra is an Assistant Professor at the Department of Life Sciences, National Institute of Technology (NIT) in Rourkela, India. She completed her Ph.D. at the University of Bremen, Germany, prior to postdoctoral studies at the School of Medicine, University of San Diego, California, USA; Department of Biological Sciences, Indiana University, Bloomington, USA; and Max Plank Institute of Cell Biology and Genetics, Dresden, Germany.

Further, she previously served as an Assistant Professor at the Department of Biological Sciences, BITS in Pilani, India from 2012-2014. Currently, her lab at the NIT Rourkela is investigating various factors that can affect the development of Drosophila’s sensory organs. The lab’s findings have been published in many prominent international journals.


Bibliographic information

  • Book Title Fundamental Approaches to Screen Abnormalities in Drosophila
  • Editors Monalisa Mishra
  • Series Title Springer Protocols Handbooks
  • Series Abbreviated Title Springer Protocols Handbooks
  • DOI https://doi.org/10.1007/978-1-4939-9756-5
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-9755-8
  • Softcover ISBN 978-1-4939-9758-9
  • eBook ISBN 978-1-4939-9756-5
  • Series ISSN 1949-2448
  • Series E-ISSN 1949-2456
  • Edition Number 1
  • Number of Pages VII, 265
  • Number of Illustrations 5 b/w illustrations, 111 illustrations in colour
  • Topics Developmental Biology
    Behavioral Sciences
    Animal Models
    Metabolomics
    Biological Microscopy
  • Buy this book on publisher's site
Industry Sectors
Biotechnology