Methods and Protocols

  • Jan R. Mead
  • Michael J. Arrowood

Part of the Methods in Molecular Biology book series (MIMB, volume 2052)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Guy Robinson, Rachel M. Chalmers
    Pages 1-10
  3. Guy Robinson, Kristin Elwin, Rachel M. Chalmers
    Pages 11-22
  4. Jeffrey W. Priest, Delynn M. Moss
    Pages 61-85
  5. Dawn M. Roellig, Lihua Xiao
    Pages 103-116
  6. Guy Robinson, Rachel M. Chalmers
    Pages 129-138
  7. Susanne Warrenfeltz, Jessica C. Kissinger, On Behalf of the EuPathDB Team
    Pages 139-192 Open Access
  8. Alejandro Castellanos-Gonzalez
    Pages 193-203
  9. Ji-Hao Xu, Nicholas W. Mathy, Xian-Ming Chen
    Pages 205-218
  10. Adam Sateriale, Mattie Pawlowic, Sumiti Vinayak, Carrie Brooks, Boris Striepen
    Pages 219-228
  11. Michael W. Riggs, Deborah A. Schaefer
    Pages 253-282
  12. Melissa S. Love, Case W. McNamara
    Pages 303-317
  13. Georgia Wilke, Yi Wang, Soumya Ravindran, Thaddeus Stappenbeck, William H. Witola, L. David Sibley
    Pages 351-372
  14. Daviel Cardenas, Seema Bhalchandra, Hymlaire Lamisere, Ying Chen, Xi-Lei Zeng, Sasirekha Ramani et al.
    Pages 373-402
  15. Susanne Warrenfeltz, Jessica C. Kissinger, On Behalf of the EuPathDB Team
    Pages C1-C1
  16. Back Matter
    Pages 403-407

About this book


This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.

The chapter “Accessing Cryptosporidium Omic and Isolate Data via” is available open access under a Creative Commons Attribution 4.0 International License via


Protozoan Intestinal tract infection Anti-cryptosporidial drugs Genotyping Oocysts CRISPR/Cas9 Host-parasite interactions

Editors and affiliations

  • Jan R. Mead
    • 1
  • Michael J. Arrowood
    • 2
  1. 1.Atlanta Veterans Affairs Medical CenterDecaturUSA
  2. 2.Division of Foodborne, Waterborne, and Environmental DiseasesNational Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and PreventionAtlantaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9747-3
  • Online ISBN 978-1-4939-9748-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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