Brain Development

Methods and Protocols

  • Simon G. Sprecher

Part of the Methods in Molecular Biology book series (MIMB, volume 2047)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Invertebrate Models

    1. Front Matter
      Pages 1-1
    2. Wanda Buzgariu, Marie-Laure Curchod, Chrystelle Perruchoud, Brigitte Galliot
      Pages 3-24
    3. Elena Perea-Atienza, Brenda Gavilán, Simon G. Sprecher, Pedro Martinez
      Pages 59-80
    4. Frank Hirth, Danielle C. Diaper
      Pages 81-96
    5. Emma L. Powell, Iris Salecker
      Pages 137-152
    6. Karolina Miszczak, Boris Egger
      Pages 153-160
    7. Cornelia Fritsch, Simon G. Sprecher
      Pages 161-189
    8. Max S. Farnworth, Kolja N. Eckermann, Hassan M. M. Ahmed, Dominik S. Mühlen, Bicheng He, Gregor Bucher
      Pages 191-217
    9. Vera S. Hunnekuhl, Janna Siemanowski, Max S. Farnworth, Bicheng He, Gregor Bucher
      Pages 233-251
    10. Jakob Krieger, Franziska Spitzner
      Pages 253-270
    11. Andreas Wanninger, Tim Wollesen
      Pages 311-324
    12. Yann Le Petillon, Stéphanie Bertrand, Héctor Escrivà
      Pages 347-359
  3. Vertebrate Models

    1. Front Matter
      Pages 361-361
    2. Kai-li Liu, Xiu-mei Wang, Zi-long Li, Ying Liu, Rong-qiao He
      Pages 363-375
    3. Jennifer E. Bestman, Hollis T. Cline
      Pages 377-395
    4. Gema Valera, Hernán López-Schier
      Pages 411-419
    5. Richard P. Tucker, Tatsuto Ishimaru, Qizhi Gong
      Pages 421-437
    6. Georgia Tsapara, Irwin Andermatt, Esther T. Stoeckli
      Pages 439-456
    7. Andrea Streit, Claudio D. Stern
      Pages 457-473
    8. Claudius F. Kratochwil, Filippo M. Rijli
      Pages 491-512
    9. Emilie Pacary, François Guillemot
      Pages 513-523
  4. Back Matter
    Pages 525-527

About this book


This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizing recent technical advances in molecular genetics for the study of the brain.


Neuronal network formation Neurotransmitter systems Genetic model systems Genetic manipulations CRISPR/Cas9 Genome editing Molecular genetics

Editors and affiliations

  • Simon G. Sprecher
    • 1
  1. 1.Department of BiologyUniversity of FribourgFribourgSwitzerland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9731-2
  • Online ISBN 978-1-4939-9732-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site