Stable Isotope Probing

Methods and Protocols

  • Marc G. Dumont
  • Marcela Hernández García

Part of the Methods in Molecular Biology book series (MIMB, volume 2046)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Zhongjun Jia, Weiwei Cao, Marcela Hernández García
    Pages 17-29
  3. Noor-Ul-Huda Ghori, Benjamin Moreira-Grez, Paton Vuong, Ian Waite, Tim Morald, Michael Wise et al.
    Pages 31-44
  4. Xavier Mayali, Peter K. Weber, Erin Nuccio, Jory Lietard, Mark Somoza, Steven J. Blazewicz et al.
    Pages 71-87
  5. Samuel E. Barnett, Nicholas D. Youngblut, Daniel H. Buckley
    Pages 109-128
  6. Egbert Schwartz, Michaela Hayer, Bruce A. Hungate, Rebecca L. Mau
    Pages 129-136
  7. Brianna K. Finley, Michaela Hayer, Rebecca L. Mau, Alicia M. Purcell, Benjamin J. Koch, Natasja C. van Gestel et al.
    Pages 137-149
  8. Ang Hu, Yahai Lu, Marcela Hernández García, Marc G. Dumont
    Pages 163-174
  9. Xiuran Yin, Ajinkya C. Kulkarni, Michael W. Friedrich
    Pages 189-206
  10. Severin Weis, Sylvia Schnell, Markus Egert
    Pages 221-231
  11. Back Matter
    Pages 245-247

About this book


This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.


SIP experiments Microbial ecology Labeled DNA analysis In silico analyses Metagenomics

Editors and affiliations

  • Marc G. Dumont
    • 1
  • Marcela Hernández García
    • 2
  1. 1.School of Biological SciencesUniversity of SouthamptonSouthamptonUK
  2. 2.School of Biological SciencesUniversity of SouthamptonSouthamptonUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9720-6
  • Online ISBN 978-1-4939-9721-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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