Purinergic Signaling

Methods and Protocols

  • Pablo Pelegrín

Part of the Methods in Molecular Biology book series (MIMB, volume 2041)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Geoffrey Burnstock
    Pages 1-15
  3. Robin M. H. Rumney, Dariusz C. Górecki
    Pages 17-43
  4. Christa E. Müller, Younis Baqi, Vigneshwaran Namasivayam
    Pages 45-64
  5. Anastasios Stavrou, Sudad Dayl, Ralf Schmid
    Pages 65-75
  6. Rebeca Padrão Amorim, Iscia Teresinha Lopes Cendes, Maria Jose da Silva Fernandes
    Pages 77-86
  7. Karolina Losenkova, Marius Paul, Heikki Irjala, Sirpa Jalkanen, Gennady G. Yegutkin
    Pages 107-116
  8. Nicole Schwarz, Marten Junge, Friedrich Haag, Friedrich Koch-Nolte
    Pages 117-136
  9. Vincent Compan, François Rassendren
    Pages 147-153
  10. Vincent Compan, François Rassendren
    Pages 155-162
  11. Mark Soave, Joëlle Goulding, Robert Markus, Stephen J. Hill, Leigh A. Stoddart
    Pages 163-181
  12. Elena De Marchi, Elisa Orioli, Anna Pegoraro, Elena Adinolfi, Francesco Di Virgilio
    Pages 183-195
  13. Kirstan A. Vessey, Tracy Ho, Andrew I. Jobling, Anna Y. Wang, Erica L. Fletcher
    Pages 209-221
  14. Klaus E. Kaschubowski, Axel E. Kraft, Viacheslav O. Nikolaev, Friedrich Haag
    Pages 223-231
  15. Laura de Diego-García, Álvaro Sebastián-Serrano, Carolina Bianchi, Caterina Di Lauro, Miguel Díaz-Hernández
    Pages 233-241
  16. Eléonore Bertin, Audrey Martínez, Eric Boué-Grabot
    Pages 243-259
  17. Carol J. Milligan, Lin-Hua Jiang
    Pages 285-300
  18. Benjamin N. Atkinson, Vijay Chudasama, Liam E. Browne
    Pages 301-309
  19. Maria Teresa Miras-Portugal, Felipe Ortega, Javier Gualix, Raquel Perez-Sen, Esmerilda G. Delicado, Rosa Gomez-Villafuertes
    Pages 311-321
  20. Ben J. Gu, Pavan Avula, James S. Wiley
    Pages 323-334
  21. Taylor J. Moon, Michael R. Elliott
    Pages 345-349
  22. Juan Jose Martínez-García, Pablo Pelegrín
    Pages 351-358
  23. Back Matter
    Pages 359-360

About this book


This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models,  in silico modeling, knock down purinoceptor expression, bioluminiscence resonance energy transfer, enzyme-based biosensors, recording P2X receptor electrophysiology, controlling P2X receptors by optogenetics, inflammasome activation, leukocyte migration, and cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Purinergic Signaling: Methods and Protocols will provide a sound basis for molecular, cellular, and physiological research into purinergic signaling in health and disease and will spark interest in this fascinating signaling process among researchers in many different and unrelated disciplines.


Pharmacology development Drug targeting Nucleotides Purinergic receptors Animal models

Editors and affiliations

  • Pablo Pelegrín
    • 1
  1. 1.BioMedical Research Institute of Murcia (IMIB)Clinical University Hospital Virgen de la ArrixacaMurciaSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9716-9
  • Online ISBN 978-1-4939-9717-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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