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Cerebrospinal Fluid (CSF) Proteomics

Methods and Protocols

  • Enrique Santamaría
  • Joaquín Fernández-Irigoyen
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2044)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Joaquín Fernández-Irigoyen, Fernando Corrales, Enrique Santamaría
      Pages 3-23
  3. Cerebrospinal Fluid Sample Preparation Methods for Proteomic Workflows

    1. Front Matter
      Pages 25-25
    2. Yanaika S. Hok-A-Hin, Eline A. J. Willemse, Charlotte E. Teunissen, Marta Del Campo
      Pages 27-50
    3. Teresa Caprile, Francisco Lamus, María Isabel Alonso, Hernán Montecinos, Angel Gato
      Pages 51-60
    4. Katalin Barkovits, Lars Tönges, Katrin Marcus
      Pages 61-67
    5. Sandra Pacharra, Katrin Marcus, Caroline May
      Pages 69-77
  4. Shotgun Cerebrospinal Fluid Proteomics

    1. Front Matter
      Pages 79-79
    2. Aishwarya A. Rao, Kanika Mehta, Nikita Gahoi, Sanjeeva Srivastava
      Pages 81-110
    3. Rustam H. Ziganshin, Sergey I. Kovalchuk, Igor V. Azarkin
      Pages 111-118
    4. Ramona Birke, Eberhard Krause, Michael Schümann, Ingolf E. Blasig, Reiner F. Haseloff
      Pages 119-128
    5. Mercedes Lachén-Montes, Andrea González-Morales, Joaquín Fernández-Irigoyen, Enrique Santamaría
      Pages 155-168
  5. Cerebrospinal Fluid Subproteomics

    1. Front Matter
      Pages 191-191
    2. Marina Gay, Ester Sánchez-Jiménez, Laura Villarreal, Mar Vilanova, Romain Huguet, Gianluca Arauz-Garofalo et al.
      Pages 193-219
    3. Alba Simats, Laura Ramiro, Joan Montaner, Teresa García-Berrocoso
      Pages 221-231
    4. Andrea González-Morales, Mercedes Lachén-Montes, Joaquín Fernández-Irigoyen, Enrique Santamaría
      Pages 233-246
    5. Angela Messina, Angelo Palmigiano, Rosaria Ornella Bua, Donata Agata Romeo, Rita Barone, Luisa Sturiale et al.
      Pages 255-272
    6. Mercedes Lachén-Montes, Andrea González-Morales, Joaquín Fernández-Irigoyen, Enrique Santamaría
      Pages 273-289
    7. Elisa Pin, Ronald Sjöberg, Eni Andersson, Cecilia Hellström, Jennie Olofsson, August Jernbom Falk et al.
      Pages 303-318
  6. Other–Omics Applied to Cerebrospinal Fluid

    1. Front Matter
      Pages 319-319
    2. Philipp Hörmann, Katalin Barkovits, Katrin Marcus, Karsten Hiller
      Pages 337-342
    3. Javier Riancho, Ana Santurtun, Pascual Sánchez-Juan
      Pages 343-352
    4. Laura Millán, Joaquín Fernández-Irigoyen, Enrique Santamaría, Rebeca Mayo
      Pages 353-361
  7. Bioinformatics Applied to Cerebrospinal Fluid

    1. Front Matter
      Pages 363-363
    2. Daimei Sasayama, Kotaro Hattori, Hiroshi Kunugi
      Pages 365-376
    3. Astrid Guldbrandsen, Yehia Mokhtar Farag, Ragnhild Reehorst Lereim, Frode S. Berven, Harald Barsnes
      Pages 377-391
    4. Fábio Trindade, Rita Nogueira-Ferreira, Paulo Bastos, Francisco Amado, Rita Ferreira, Rui Vitorino
      Pages 393-553
  8. Back Matter
    Pages 555-557

About this book

Introduction

This volume focuses on protein analysis, including a wide range of the use of mass spectrometry and other protein methods within neurobiological disciplines. Chapters cover topics such as cerebrospinal fluid (CSF) processing and biobanking; label-free quantitative proteomics; SWATH; top-down proteomics; and experimental strategies based on other –omics applied to CSF metabolome, lipidome, and microRNAome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and thorough, Cerebrospinal Fluid (CSF) Proteomics: Methods and Protocols is a valuable resource for graduate students and post-doctoral fellows interested in learning more about CSF proteotyping. It is also useful to established researchers seeking further insight into this growing field. 

Keywords

neuroinflammation Olink technology biomarkers QPRESTs endopeptidome

Editors and affiliations

  • Enrique Santamaría
    • 1
  • Joaquín Fernández-Irigoyen
    • 2
  1. 1.Proteomics Unit, Clinical Neuroproteomics Laboratory, Navarrabiomed, Complejo Hospitalario de Navarra (CHN)Universidad Pública de Navarra (UPNA), IdiSNA, Proteored-ISCIIIPamplonaSpain
  2. 2.Proteomics Unit, Clinical Neuroproteomics Laboratory, Navarrabiomed, Complejo Hospitalario de Navarra (CHN)Universidad Pública de Navarra (UPNA), IdiSNA, Proteored-ISCIIIPamplonaSpain

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-9706-0
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9705-3
  • Online ISBN 978-1-4939-9706-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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