Chlamydia trachomatis

Methods and Protocols

  • Amanda Claire Brown

Part of the Methods in Molecular Biology book series (MIMB, volume 2042)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Tomislav Meštrović, Dezső P. Virok, Sunčanica Ljubin-Sternak, Tímea Raffai, Katalin Burián, Jasmina Vraneš
    Pages 33-43
  3. Amanda Claire Brown, Mette T. Christiansen
    Pages 45-67
  4. Martina Jelocnik, Adam Polkinghorne, Yvonne Pannekoek
    Pages 69-86
  5. James W. Marsh, Regan J. Hayward, Amol Shetty, Anup Mahurkar, Michael S. Humphrys, Garry S. A. Myers
    Pages 123-135
  6. Oleg V. Podgorny, Nadezhda F. Polina, Vassili N. Lazarev
    Pages 137-150
  7. Katerina Wolf, Mostafa Rahnama, Kenneth A. Fields
    Pages 151-164
  8. Mary M. Weber, Robert Faris
    Pages 165-184
  9. Mateusz Kędzior, Robert J. Bastidas
    Pages 185-204
  10. Rachel Ende, Isabelle Derré
    Pages 205-218
  11. Charles W. Armitage, Alison J. Carey, Kenneth W. Beagley
    Pages 219-236
  12. Macy G. Olson, Lisa M. Jorgenson, Ray E. Widner, Elizabeth A. Rucks
    Pages 245-278
  13. Back Matter
    Pages 287-289

About this book


This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and descriptions of genotyping technologies, as well as dual RNA-Seq transcriptional profiling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative, practical, and relevant, Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs.


Drug resistance Bacterial pathogens Bacterium biology Drug discovery Treatment regimens C. trachomatis

Editors and affiliations

  • Amanda Claire Brown
    • 1
  1. 1.Department of Microbiology and Immunology, Vet SchoolCornell UniversityIthacaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9693-3
  • Online ISBN 978-1-4939-9694-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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