Methods and Protocols

  • Sam Massa
  • Nick Devoogdt

Part of the Methods in Molecular Biology book series (MIMB, volume 2033)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Stephan Dickgiesser, Roland Kellner, Harald Kolmar, Nicolas Rasche
    Pages 1-14
  3. Maurício Morais, Nafsika Forte, Vijay Chudasama, James R. Baker
    Pages 15-24
  4. Maria J. Matos, Gonzalo Jiménez-Osés, Gonçalo J. L. Bernardes
    Pages 25-37
  5. Rania A. Hashad, Jaclyn L. Lange, Natasha C. W. Tan, Karen Alt, Christoph E. Hagemeyer
    Pages 67-80
  6. Makan Khoshnejad, Jacob S. Brenner, Hamideh Parhiz, Vladimir R. Muzykantov
    Pages 81-93
  7. Sandra Liebscher, Frank Bordusa
    Pages 95-115
  8. Geert-Jan Graulus, Duy Tien Ta, Huong Tran, Rebekka Hansen, Brecht Billen, Erik Royackers et al.
    Pages 117-130
  9. Karin F. K. Ejendal, Julia G. Fraseur, Tamara L. Kinzer-Ursem
    Pages 149-165
  10. Dominik Schumacher, Heinrich Leonhardt, Christian P. R. Hackenberger, Jonas Helma
    Pages 167-189
  11. Christopher R. Drake, Michael J. Evans, Henry F. VanBrocklin
    Pages 191-205
  12. Yi Zhang, Shelby Auger, Jonas V. Schaefer, Andreas Plückthun, Mark D. Distefano
    Pages 207-219
  13. Feng Tang, Wei Shi, Wei Huang
    Pages 221-238
  14. Partha Sarathi Addy, Sarah B. Erickson, James S. Italia, Abhishek Chatterjee
    Pages 239-251
  15. Hannes Merten, Jonas V. Schaefer, Fabian Brandl, Uwe Zangemeister-Wittke, Andreas Plückthun
    Pages 253-273
  16. Arutselvan Natarajan, Lotfi Abou-Elkacem
    Pages 301-313
  17. Back Matter
    Pages 315-317

About this book


This book explores well-established and emerging conjugation strategies that are relevant for proteins used in the field of precision medicine, focusing on techniques that are suitable for antibodies, antibody-fragments such as Fabs, scFvs, or nanobodies, scaffold proteins such as FN3 or DARPin, peptides, or model proteins. Although centered on the development of bioconjugates rather than their application, most protocols also show the conjugation of the targeting vehicle to a diagnostic or therapeutic entity, with the end-product most often being an antibody-drug conjugate, an optical probe, a nanomedicine, or a radiopharmaceutical. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Bioconjugation: Methods and Protocols is an ideal guide for researchers looking toward precision medicine in order to expand the vital field of drug discovery.


Personalized medicine Drug discovery Antibodies Antibody fragments Targeting agents Precision medicine Therapeutic compounds Scaffold proteins Antibody-drug conjugates

Editors and affiliations

  • Sam Massa
    • 1
  • Nick Devoogdt
    • 2
  1. 1.Cellular and Molecular Immunology LaboratoryVrije Universiteit BrusselBrusselsBelgium
  2. 2.In Vivo Cellular and Molecular Imaging Laboratory (ICMI)Vrije Universiteit BrusselBrusselsBelgium

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9653-7
  • Online ISBN 978-1-4939-9654-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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