Amino Acid Analysis

Methods and Protocols

  • Michail A. Alterman

Part of the Methods in Molecular Biology book series (MIMB, volume 2030)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Mathilde Louwagie, Sylvie Kieffer-Jaquinod, Virginie Brun
    Pages 1-10
  3. Olga A. Mirgorodskaya, Roman Körner, Yuri P. Kozmin, Peter Roepstorff
    Pages 11-16
  4. Tobias Langrock, Ralf Hoffmann
    Pages 47-56
  5. Jennifer A. Tripp, Thibaut Devièse, James S. O. McCullagh
    Pages 69-83
  6. Zhaolai Dai, Shiqiang Sun, Hui Chen, Moyan Liu, Lianhua Zhang, Zhenlong Wu et al.
    Pages 131-142
  7. Giuseppe Giordano, Antonina Gucciardi, Paola Pirillo, Mauro Naturale
    Pages 153-172
  8. Katja Dettmer, Axel P. Stevens, Stephan R. Fagerer, Hannelore Kaspar, Peter J. Oefner
    Pages 173-190
  9. Yosuke Nakano, Moyu Taniguchi, Yutaka Umakoshi, Daisuke Watai, Eiichiro Fukusaki
    Pages 253-261
  10. María Castro-Puyana, María Luisa Marina
    Pages 277-291
  11. Akiyoshi Hirayama, Satsuki Ikeda, Asako Sato, Tomoyoshi Soga
    Pages 307-313
  12. Ine Rombouts, Bert Lagrain, Lieve Lamberts, Inge Celus, Kristof Brijs, Jan A. Delcour
    Pages 381-394
  13. Björn Thiele, Michelle Hupert, Beatrix Santiago-Schübel, Marco Oldiges, Diana Hofmann
    Pages 403-414
  14. Ward van Helmond, Vincent O’Brien, Robin de Jong, Jan van Esch, Sander Oldenhof, Marcel de Puit
    Pages 429-438
  15. Back Matter
    Pages 451-455

About this book


Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols, Second Edition presents a broad spectrum of all available methods allowing readers to choose the method that best suits their particular laboratory set-up and analytical needs. In this volume, readers can find chapters describing general, as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry, as well as in food analysis, microbiology, marine biology, drug metabolism, even archaeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Amino Acid Analysis: Methods and Protocols, Second Edition provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.


Metabolic Phenotypes RP-HPLC Mass Spectrometry Phenylthiocarbamyl Amino Acid Adducts Liquid Chromatography

Editors and affiliations

  • Michail A. Alterman
    • 1
  1. 1.Office of Policy for Pharmaceutical Quality Office of Pharmaceutical QualityCenter for Drug Evaluation and Research US FDASilver SpringUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9638-4
  • Online ISBN 978-1-4939-9639-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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