© 2019

Antiviral Resistance in Plants

Methods and Protocols

  • Kappei Kobayashi
  • Masamichi Nishiguchi
  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 2028)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Kappei Kobayashi, Reiko Tomita, Ken-Taro Sekine
    Pages 11-25
  3. Kengo Idehara, Reiko Tomita, Ken-Taro Sekine, Masamichi Nishiguchi, Kappei Kobayashi
    Pages 97-113
  4. Naoi Hosoe, Takuya Keima, Yuji Fujimoto, Yuka Hagiwara-Komoda, Masayoshi Hashimoto, Kensaku Maejima et al.
    Pages 145-151
  5. Zhila Osmani, Shinnosuke Jin, Masafumi Mikami, Masaki Endo, Hiroki Atarashi, Kaien Fujino et al.
    Pages 153-165
  6. Zahir Ali, Syed Shan-e-Ali Zaidi, Manal Tashkandi, Magdy M. Mahfouz
    Pages 167-183
  7. Masamichi Nishiguchi, Emran Md. Ali, Hui Chen, Masayuki Ishikawa, Kappei Kobayashi
    Pages 247-259
  8. Chunjiang Li, Noriko Yamagishi, Nobuyuki Yoshikawa
    Pages 273-288
  9. Back Matter
    Pages 289-292

About this book


This detailed book explores strategies that have been developed to combat plant virus infection. Beginning with a section on techniques for identifying and studying the virus resistance gene involved in plant innate immunity, the volume continues by delving into techniques related to novel mechanisms of plant virus resistance, methods for the analysis and practical use of RNA silencing, as well as methods for the development of plant viral vaccines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols serves as an ideal guide for researchers working to combat the serious threats plant virus diseases represent for agricultural production and beyond.


Plant virus infection Plant innate immunity Viral resistance mechanisms RNA silencing Vaccines Infection pathways Plant genetics

Editors and affiliations

  • Kappei Kobayashi
    • 1
  • Masamichi Nishiguchi
    • 2
  1. 1.Faculty of AgricultureEhime UniversityMatsuyamaJapan
  2. 2.Faculty of AgricultureEhime UniversityMatsuyamaJapan

Bibliographic information

  • Book Title Antiviral Resistance in Plants
  • Book Subtitle Methods and Protocols
  • Editors Kappei Kobayashi
    Masamichi Nishiguchi
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-9634-6
  • Softcover ISBN 978-1-4939-9637-7
  • eBook ISBN 978-1-4939-9635-3
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 292
  • Number of Illustrations 10 b/w illustrations, 27 illustrations in colour
  • Topics Plant Sciences
  • Buy this book on publisher's site
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